Browsing by Author "Rodriguez-Marconi, Susana"

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    Distinct oxygen environments shape picoeukaryote assemblages thriving oxygen minimum zone waters off central Chile
    (2020) De la Iglesia, Rodrigo; Echenique-Subiabre, Isidora; Rodriguez-Marconi, Susana; Pablo Espinoza, Juan; von Dassow, Peter; Ulloa, Osvaldo; Trefault, Nicole
    Oxygen minimum zones (OMZs) support ocean biogeochemical cycles of global importance. The OMZ off central Chile is characterized by seasonally variable oxygen concentrations due to upwelling events. Bacterial and archaeal communities from this area have been previously described; however, picoeukaryote communities remain largely unexplored. In order to improve our knowledge on picoeukaryote ecology and the effect of controlling factors on its community structure, environmental parameters and 18S rRNA metabarcoding analyses were performed in water samples collected at several depths at a time series station on the continental shelf in March, May and August. Our results showed that oxygen, nitrate, silicate and temperature are relevant factors shaping the picoeukaryote community structure. Overall, according to our sequence dataset, the OMZ was dominated by Dinophyceae members including marine parasitic dinoflagellates. Moreover, dysoxic and suboxic conditions were enriched by fungi and phagotrophic protists from Ustilaginomycetes, Bicoecea and Choanoflagellatea. The latter is particularly relevant in the understanding of metazoan evolution and the origins of multicellularity in low-oxygen environments. Picoeukaryote communities changed significantly over the 3 months sampled with variations in water column stratification, including the occurrence of a winter bloom of Mamiellales. Altogether, this study reveals a great diversity and dynamics of picoeukaryotes inhabiting a coastal OMZ.
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    Unveiling microbial guilds and symbiotic relationships in Antarctic sponge microbiomes
    (2024) Moreno-Pino, Mario; Manrique-de-la-Cuba, Maria F.; Lopez-Rodriguez, Marileyxis; Parada-Pozo, Genesis; Rodriguez-Marconi, Susana; Ribeiro, Catherine Gerikas; Flores-Herrera, Patricio; Guajardo, Mariela; Trefault, Nicole
    Marine sponges host diverse microbial communities. Although we know many of its ecological patterns, a deeper understanding of the polar sponge holobiont is still needed. We combine high-throughput sequencing of ribosomal genes, including the largest taxonomic repertoire of Antarctic sponge species analyzed to date, functional metagenomics, and metagenome-assembled genomes (MAGs). Our findings show that sponges harbor more exclusive bacterial and archaeal communities than seawater, while microbial eukaryotes are mostly shared. Furthermore, bacteria in Antarctic sponge holobionts establish more cooperative interactions than in sponge holobionts from other environments. The bacterial classes that established more positive relations were Bacteroidia, Gamma- and Alphaproteobacteria. Antarctic sponge microbiomes contain microbial guilds that encompass ammonia-oxidizing archaea, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, and sulfur-oxidizing bacteria. The retrieved MAGs showed a high level of novelty and streamlining signals and belong to the most abundant members of the main microbial guilds in the Antarctic sponge holobiont. Moreover, the genomes of these symbiotic bacteria contain highly abundant functions related to their adaptation to the cold environment, vitamin production, and symbiotic lifestyle, helping the holobiont survive in this extreme environment.