Browsing by Author "Rodriguez, M"

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    Antibiotic-resistant Salmonella typhi from two outbreaks: Few ribotypes and IS200 types harbor Inc HI1 plasmids
    (1997) Fica, A; Fernandez-Beros, ME; Aron-Hott, L; Rivas, A; D'Ottone, K; Chumpitaz, J; Guevara, JM; Rodriguez, M; Cabello, F
    To investigate factors that could be involved in the emergence of antibiotic resistant S. typhi, we characterized R plasmids and antibiotic resistant S. typhi strains from two outbreaks of typhoid in Peru and Chile. Differences in the Inc HI1 plasmids of Peruvian and Chilean strains were identified by conjugation and incompatibility studies and plasmid DNA characterization. Antibiotic-resistant S. typhi harboring Inc HI1 plasmids belonged to a reduced number of Pst1 and Cla1 ribotypes and IS200 types, in contrast to the high genetic diversity found among epidemic antibiotic-susceptible S. typhi.(15) The low diversity of antibiotic-resistant S. typhi suggests that they may express properties that are related to both their ability to harbor Inc HI1 R plasmids and to disseminate.
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    Determination of a BRCA1 gene mutation in a family with hereditary breast cancer
    (SOC MEDICA SANTIAGO, 2004) Gallardo, M; Faundez, P; Cruz, A; Rodriguez, M; Alvarez, M; Carvallo, P
    Background: Breast cancer is the main cause of death among fore, the women between 40 and 55 years old, in whom the hereditary cases are common. Therefore molecular diagnosis of germ line initiations involved in breast cancer susceptibilily is relevant. BRCA1 and BRCA2 have been described as the two major genes involved in familial breast/ovarian cancer. We are performing a screening for BRCA 1 and BRCA2 genes, in a group of 50 high risk Chilean families for breast/ovarian cancer. We have detected a mutation, 3936 C > T, that leads to a truncated protein, in two affected women from one of the families in study.
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    Incidence of BRCA1 and BRCA2 mutations in 54 Chilean families with breast/ovarian cancer, genotype-phenotype correlations
    (2006) Gallardo, M; Silva, A; Rubio, L; Alvarez, C; Torrealba, C; Salinas, M; Tapia, T; Faundez, P; Palma, L; Riccio, M; Paredes, H; Rodriguez, M; Cruz, A; Rousseau, C; King, M; Camus, M; Alvarez, M; Carvallo, P
    Our aim was to analyze the incidence of mutations in BRCA1 and BRCA2 genes in 54 families with breast/ovarian cancer. Families were selected from three Institutions following the standard criteria for hereditary breast/ovarian cancer. PCR amplification of all exons was performed, followed by SSCP, heteroduplex, PTT and sequencing analysis. We identified eight truncation mutations, three in the BRCA1 gene and five in the BRCA2 gene. Three of these mutations have not been reported previously by other groups: 308insA in one family, 3936 C > T in two families, for BRCA1, and 4970insTG in one family for BRCA2. In addition two families having Ashkenazi Jewish ancestors present the well known mutations 185delAG and 6174delT. Interestingly, 5 out of 11 families have mutations recurrent in Spanish families. Among the 54 families selected, seven have breast and ovary cancer cases, and only two presented a mutation in BRCA1 or BRCA2 genes. Other cancers as prostate and stomach are frequent among relatives carrying the mutation. Five cases of very early onset (< 31 years old) breast cancer were detected. The frequencies of BRCA1 (0.074) and BRCA2 (0.13) mutations in our families is low but similar to the incidence found in other populations, like in Spain. Since is widely known that risk factors that modulate the development of breast cancer such as lifestyle risk factors, geographic location, country of origin and socioeconomic status, besides a familial history of breast cancer our findings suggest that the history of colonization and immigrations is very relevant when studying hereditary factors associated to breast cancer.
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    Interoceptive Insular Cortex Mediates Both Innate Fear and Contextual Threat Conditioning to Predator Odor
    (2020) Rodriguez, M; Ceric Garrido, Francisco José; Murgas, P; Harland, B; Torrealba, Fernando; Contreras, M