Browsing by Author "Rios Bustamante, Juan Carlos"

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    Alpha-Lipoic Acid-Mediated Inhibition of LTB4 Synthesis Suppresses Epithelial-Mesenchymal Transition, Modulating Functional and Tumorigenic Capacities in Non-Small Cell Lung Cancer A549 Cells
    (Elsevier Inc., 2025) Torres Torres, María José; Rios Bustamante, Juan Carlos; Valle Miranda, Alexandra Belén; Indo, Sebastián; Brockway Valenzuela, Kevin Sebastian; Lopez-Moncada, Fernanda; Faundez Cáceres, Mario Antonio; Castellón, Enrique A.; Contreras, Héctor R.
    Background: Leukotriene B4 (LTB4) plays a crucial role in carcinogenesis by inducing epithelial-mesenchymal transition (EMT), a process associated with tumor progression. The synthesis of LTB4 is mediated by leukotriene A4 hydrolase (LTA4H), and it binds to the receptors BLT1 and BLT2. Dysregulation in LTB4 production is linked to the development of various pathologies. Therefore, the identification or design of inhibitors of LTB4 synthesis or receptor antagonists represents an ongoing challenge. In this context, our laboratory previously demonstrated that alpha-lipoic acid (ALA) inhibits LTA4H. The objective of this study was to evaluate the effect of ALA on the expression of canonical EMT markers and the functional and tumorigenic capacities induced by LTB4 in A549 cells. Methods: The expression of cPLA2, 5LOX, FLAP, LTA4H, BLT1, and LTB4 production in human adenocarcinomic alveolar basal epithelial A549 cells was assessed using Western blot, RT-qPCR, and ELISA, respectively. Subsequently, the expression of canonical EMT markers was evaluated by Western blot. Functional assays were performed to assess cell viability, proliferation, invasion, migration, and clonogenicity using MTT, Western blot, Transwell assays, and colony formation assays, respectively. Results were expressed as median with interquartile range (n≥3) and analyzed using the Kruskal-Wallis or Tukey multiple comparisons tests. Results: A549 cells express key proteins involved in LTB4 synthesis and receptor binding, including LTA4H and BLT1, and ALA inhibits the production of LTB4. Additionally, LTA4H and BLT1 were detected in lung adenocarcinoma tissue samples. LTB4 was found to induce EMT, whereas ALA treatment enhanced the expression of epithelial markers and reduced the expression of mesenchymal markers. Furthermore, ALA treatment resulted in a decrease in LTB4 levels and attenuated the functional and tumorigenic capacities of A549 cells, including their viability, migration, invasion, and clonogenic potential. Conclusions: These findings suggest that ALA may offer therapeutic potential in the context of lung cancer, as it could be integrated into conventional pharmacological therapies to enhance treatment efficacy and mitigate the adverse effects associated with chemotherapy. Further studies are warranted to confirm the clinical applicability of ALA as an adjunctive treatment in lung cancer.
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    Characterization of exposures in preschoolers reported to a Chilean Poison Control Center in 2020
    (TAYLOR & FRANCIS LTD, 2023) Sateler Villanueva, Antonia; Bettini Silva, Marli Francesca; Silva Peña, Lorena Alejandra; Medel Jara, Patricio Andres; Iturra Montecinos, Pablo Andres; Figueroa Silva, Francisco Julio Antonio; Irarrazabal Vargas, Lisette Paola; Rios Bustamante, Juan Carlos
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    Data harmonization: Comparative study of four poison centres using INTOX data management system software
    (Springer, 2010) Rios Bustamante, Juan Carlos; Fruchtengarten, L.; Meneses, C.; Lynch, A. M.; Tempowski, J.; Bettini, M.; Mieres Araya, Juan José; Zucollo, A.; Paris, Enrique
    Introduction: The nurse-patient relationship recognizes the dignity of people and their right to respectful care during health care. The preceptor is essential in the formation of nurses, is a model and guide in the processes of approximation to the professional work, facilitates learning and is a link between theory and practice. The manuscript describes the competences of the nursing preceptor for the training of students who promote respectful care in health care. Material and method: Qualitative descriptive study with focal groups with nursing preceptor. Data analysis according to Kyngäs. Results: The analysis allowed to distinguish 4 categories relatives to Clinical teaching competence: a) Course development, b) Creation of safe learning environments, c) Patient-centered care, and d) Interpersonal relational skills. Conclusion: It is the responsibility of the institutions to train clinical preceptor in respectful care according to values and professional profile.
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    Data harmonization: Comparative study of four poison centres using INTOX data management system software
    (ELSEVIER IRELAND LTD, 2010) Rios Bustamante, Juan Carlos; Fruchtengarten, L.; Meneses, C.; Lynch, A. M.; Tempowski, J.; Bettini, M.; Mieres Araya, Juan José; Zucollo, A.; Paris, Enrique