Browsing by Author "Reimann, Carolin"

Now showing 1 - 6 of 6
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    Assessment of Albumin ECM Accumulation and Inflammation as Novel In Vivo Diagnostic Targets for Multi-Target MR Imaging
    (2021) Möckel, Jana; Brangsch, Julia; Reimann, Carolin; Kaufmann, Jan O.; Sack, Ingolf; Mangarova, Dilyana B.; Avan, Kader; Taupitz, Matthias; Adams, Lisa C.; Keller, Sarah; Antje, Ludwig; Hamm, Bernd; Botnar, René Michael; Makowski, Marcus R.
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    Concurrent Molecular Magnetic Resonance Imaging of Inflammatory Activity and Extracellular Matrix Degradation for the Prediction of Aneurysm Rupture
    (2019) Brangsch, Julia; Reimann, Carolin; Kaufmann, Jan O.; Adams, Lisa C.; Onthank, David C.; Thone-Reineke, Christa; Robinson, Simon P.; Buchholz, Rebecca; Karst, Uwe; Botnar, Rene M.; Hamm, Bernd; Makowski, Marcus R.
    BACKGROUND: Molecular magnetic resonance imaging is a promising modality for the characterization of abdominal aortic aneurysms (AAAs). The combination of different molecular imaging biomarkers may improve the assessment of the risk of rupture. This study investigates the feasibility of imaging inflammatory activity and extracellular matrix degradation by concurrent dual-probe molecular magnetic resonance imaging in an AAA mouse model.
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    Contrast-Enhanced Magnetic Resonance Angiography Using a Novel Elastin-Specific Molecular Probe in an Experimental Animal Model
    (2018) Reimann, Carolin; Brangsch, Julia; Kaufmann, Jan Ole; Adams, Lisa C.; Onthank, David C.; Robinson, Simon P.; Botnar, René Michael; Collettini, Federico; Makowski, Marcus R.
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    Dual-probe molecular MRI for the in vivo characterization of atherosclerosis in a mouse model : Simultaneous assessment of plaque inflammation and extracellular-matrix remodeling
    (2019) Reimann, Carolin; Brangsch, Julia; Kaufmann, Jan Ole; Adams,Lisa C.; Onthank, David C.; Thöne Reineke, Christa; Robinson, Simon P.; Hamm, Bernd; Botnar, René Michael; Makowski, Marcus R.
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    Elastin-specific MR probe for visualization and evaluation of an interleukin-1β targeted therapy for atherosclerosis
    (2024) Mangarova, Dilyana Branimirova; Reimann, Carolin; Kaufmann, Jan Ole; Moeckel, Jana; Kader, Avan; Adams, Lisa Christine; Ludwig, Antje; Onthank, David; Robinson, Simon; Karst, Uwe; Helmer, Rebecca; Botnar, Rene; Hamm, Bernd; Makowski, Marcus Richard; Brangsch, Julia
    Atherosclerosis is a chronic inflammatory condition of the arteries and represents the primary cause of various cardiovascular diseases. Despite ongoing progress, finding effective anti-inflammatory therapeutic strategies for atherosclerosis remains a challenge. Here, we assessed the potential of molecular magnetic resonance imaging (MRI) to visualize the effects of 01BSUR, an anti-interleukin-1 beta monoclonal antibody, for treating atherosclerosis in a murine model. Male apolipoprotein E-deficient mice were divided into a therapy group (01BSUR, 2 x 0.3 mg/kg subcutaneously, n = 10) and control group (no treatment, n = 10) and received a high-fat diet for eight weeks. The plaque burden was assessed using an elastin-targeted gadolinium-based contrast probe (0.2 mmol/kg intravenously) on a 3 T MRI scanner. T1-weighted imaging showed a significantly lower contrast-to-noise (CNR) ratio in the 01BSUR group (pre: 3.93042664; post: 8.4007067) compared to the control group (pre: 3.70679168; post: 13.2982156) following administration of the elastin-specific MRI probe (p < 0.05). Histological examinations demonstrated a significant reduction in plaque size (p < 0.05) and a significant decrease in plaque elastin content (p < 0.05) in the treatment group compared to control animals. This study demonstrated that 01BSUR hinders the progression of atherosclerosis in a mouse model. Using an elastin-targeted MRI probe, we could quantify these therapeutic effects in MRI.
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    Molecular MR-Imaging for Noninvasive Quantification of the Anti-Inflammatory Effect of Targeting Interleukin-1β in a Mouse Model of Aortic Aneurysm
    (SAGE Publications Inc., 2020) Brangsch, Julia; Reimann, Carolin; Kaufmann, Jan Ole; Adams, Lisa Christine; Hamm, Bernd; Makowski, Marcus Richard; Thöne-Reineke, Christa; Wilke, Marco; Weller, Michael; Onthank, David; Robinson, Simon; Buchholz, Rebecca; Karst, Uwe; Botnar, Rene Michael
    Molecular-MRI is a promising imaging modality for the assessment of abdominal aortic aneurysms (AAAs). Interleukin-1β (IL-1β) represents a new therapeutic tool for AAA-treatment, since pro-inflammatory cytokines are key-mediators of inflammation. This study investigates the potential of molecular-MRI to evaluate therapeutic effects of an anti-IL-1β-therapy on AAA-formation in a mouse-model. Methods: Osmotic-minipumps were implanted in apolipoprotein-deficient-mice (N = 27). One group (Ang-II+01BSUR group, n = 9) was infused with angiotensin-II (Ang-II) for 4 weeks and received an anti-murine IL-1β-antibody (01BSUR) 3 times. One group (Ang-II-group, n = 9) was infused with Ang-II for 4 weeks but received no treatment. Control-group (n = 9) was infused with saline and received no treatment. MR-imaging was performed using an elastin-specific gadolinium-based-probe (0.2 mmol/kg). Results: Mice of the Ang-II+01BSUR-group showed a lower aortic-diameter compared to mice of the Ang-II-group and control mice (p < 0.05). Using the elastin-specific-probe, a significant decrease in elastin-destruction was observed in mice of the Ang-II+01BSUR-group. In vivo MR-measurements correlated well with histopathology (y = 0.34x-13.81, R2 = 0.84, p < 0.05), ICP-MS (y = 0.02x+2.39; R2 = 0.81, p < 0.05) and LA-ICP-MS. Immunofluorescence and western-blotting confirmed a reduced IL-1β-expression. Conclusions: Molecular-MRI enables the early visualization and quantification of the anti-inflammatory-effects of an IL-1β-inhibitor in a mouse-model of AAAs. Responders and non-responders could be identified early after the initiation of the therapy using molecular-MRI.