Browsing by Author "Ravussin, Eric"
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- ItemAdipose tissue expression of adipose (WDTC1) gene is associated with lower fat mass and enhanced insulin sensitivity in humans(2013) Galgani Fuentes, José; Kelley, David E.; Albu, Jeanine B.; Krakoff, Jonathan; Smith, Steven R.; Bray, George A.; Ravussin, Eric
- ItemContribution of brown adipose tissue to human energy metabolism(2019) Fernández Verdejo, Rodrigo; Marlatt, Kara L.; Ravussin, Eric; Galgani Fuentes, José
- ItemEffect of an acute long-duration exercise bout on skeletal muscle lipid droplet morphology, GLUT 4 protein, and perilipin protein expression(2023) Bajpeyi, Sudip; Apaflo, Jehu N.; Rosas, Victoria; Sepulveda-Rivera, Keisha; Varela-Ramirez, Armando; Covington, Jeffrey D.; Galgani, Jose E.; Ravussin, EricPurposeSmaller lipid droplet morphology and GLUT 4 protein expression have been associated with greater muscle oxidative capacity and glucose uptake, respectively. The main purpose of this study was to determine the effect of an acute long-duration exercise bout on skeletal muscle lipid droplet morphology, GLUT4, perilipin 3, and perilipin 5 expressions.MethodsTwenty healthy men (age 24.0 & PLUSMN; 1.0 years, BMI 23.6 & PLUSMN; 0.4 kg/m(2)) were recruited for the study. The participants were subjected to an acute bout of exercise on a cycle ergometer at 50% VO(2)max until they reached a total energy expenditure of 650 kcal. The study was conducted after an overnight fast. Vastus lateralis muscle biopsies were obtained before and immediately after exercise for immunohistochemical analysis to determine lipid, perilipin 3, perilipin 5, and GLUT4 protein contents while GLUT 4 mRNA was quantified using RT-qPCR.ResultsLipid droplet size decreased whereas total intramyocellular lipid content tended to reduce (p = 0.07) after an acute bout of endurance exercise. The density of smaller lipid droplets in the peripheral sarcoplasmic region significantly increased (0.584 & PLUSMN; 0.04 to 0.638 & PLUSMN; 0.08 AU; p = 0.01) while larger lipid droplets significantly decreased (p < 0.05). GLUT4 mRNA tended to increase (p = 0.05). There were no significant changes in GLUT 4, perilipin 3, and perilipin 5 protein levels.ConclusionThe study demonstrates that exercise may impact metabolism by enhancing the quantity of smaller lipid droplets over larger lipid droplets.
- ItemMetabolic flexibility to lipid availability during exercise is enhanced in individuals with high insulin sensitivity(2018) Fernández Verdejo, Rodrigo; Bajpeyi, Sudip; Ravussin, Eric; Galgani Fuentes, José
- ItemSkeletal Muscle Perilipin 3 and Coatomer Proteins Are Increased following Exercise and Are Associated with Fat Oxidation(2014) Covington, Jeffrey D.; Galgani Fuentes, José; Moro, Cedric; La Grange, Jamie M.; Zhang, Zhengyu; Rustan, Arild C.; Ravussin, Eric; Bajpeyi, Sudip