Browsing by Author "RODRIGUEZ, L"
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- ItemADAPTIVE-CHANGES OF HEPATIC BILE-SALT TRANSPORT IN A MODEL OF REVERSIBLE INTERRUPTION OF THE ENTEROHEPATIC CIRCULATION IN THE RAT(1993) ACCATINO, L; HONO, J; KOENIG, C; PIZARRO, M; RODRIGUEZ, LThe reversibility and time course of the adaptive changes in hepatic bile salt transport related to modifications of the bile salt enterohepatic circulation and bile salt pool size have not been previously studied. For this reason a model of reversible interruption of entero-hepatic circulation was characterized in unrestrained rats, which allowed the study of changes in hepatic bile salt transport following bile salt pool depletion and subsequent restoration by either the de novo synthesis of bile acids or i.v. administration of exogenous taurocholate. Rats subjected to biliary drainage for 24 h through a transduodenal common bile duct cannula, followed by removal of the cannula and restoration of the enterohepatic circulation were studied at 24, 48 and 72 h. Neither light and electron microscopy examination nor plasma biochemical parameters showed evidence of necrosis, fibrosis, cholestasis or inflammatory changes. Maximum taurocholate secretory rate decreased to 50% following 24-h bile salt depletion. After restoration of the enterohepatic circulation maximum taurocholate secretory rate progressively increased to normal values at 72 h, following the normalization of the bile salt pool size, which had a similar composition compared with controls. The same effect was obtained when the native bile salt pool was substituted with exogenous taurocholate. Thus, adaptive down-regulation of hepatic bile salt transport capacity is a reversible process, related to restoration of entero-hepatic circulation and normalization of bile salt pool size.
- ItemCYCLOSPORINE BLOOD LEVEL MEASUREMENTS WITH MONOCLONAL AND POLYCLONAL ANTIBODIES DURING CLINICAL RENAL-TRANSPLANTATION(1989) FORADORI, AC; MARTINEZ, L; LOVELUCK, A; CORREA, L; RODRIGUEZ, L
- ItemDIAGNOSIS OF TYPHOID-FEVER BY 2 SEROLOGIC METHODS - ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF ANTILIPOPOLYSACCHARIDE OF SALMONELLA-TYPHI ANTIBODIES AND WIDAL TEST(1992) QUIROGA, T; GOYCOOLEA, M; TAGLE, R; GONZALEZ, F; RODRIGUEZ, L; VILLARROEL, LSerum samples from 85 patients with proven typhoid fever, 11 patients with p-typhoidal fever, 101 patients with febrile non-typhoidal, and 130 healthy subjects were tested for immunoglobulin G (IgG), IgA, and IgM antilipopolysaccharide (LPS) of Salmonella typhi antibodies by enzyme-linked immunosorbent assay (ELISA) and Widal test. The levels of all three classes of immunoglobulin anti-LPS of S. typhi were higher in typhoid patients than in healthy or febrile nontyphoidal groups; we selected various combinations between the three classes of immunoglobulin to obtain the best combination of sensitivity and specificity. The sum of the absorbance values obtained from the ELISA assay for IgG + IgA + IgM (SIGMAlgs) was the best choice for diagnostic utility for typhoid fever. We selected a positive test at a decision level of SIGMAlgs greater-than-or-equal-to 1.2 with a sensitivity of 94% and a specificity of 92% with a frequency of false negative of 5.9%. The frequency of false positives for healthy controls was 7.7% and, for the febrile nontyphoidal group, it was 7.9%. We also compared receiver (or relative) operating characteristic (ROC) curves for the diagnostic usefulness of the ELISA with that of the Widal test, whose merits and limitations, especially in endemic regions, are discussed. The ELISA assay was much more sensitive and specific than any combination of the Widal test, and hence it could be a useful tool for the serologic diagnosis of typhoidal fever with a single blood sample.
- ItemHLA-ANTIGEN EXPRESSION AND PANEL REACTIVE ANTIBODIES(APPLETON & LANGE, 1995) RODRIGUEZ, L; CALDUMBIDE, I; MARTINEZ, L; SCAGLIOTTI, P; QUIROGA, T
- ItemMONITORING OF CYCLOSPORINE BLOOD-LEVELS WITH POLYCLONAL AND MONOCLONAL ASSAYS DURING EPISODES OF RENAL GRAFT DYSFUNCTION(1989) MARTINEZ, L; FORADORI, A; VACCAREZZA, A; MARTINEZ, P; RODRIGUEZ, L
- ItemNOVEL GENETIC-MARKERS OF RHEUMATOID-ARTHRITIS IN CHILEAN PATIENTS, BY DR SEROTYPING AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS(1992) GONZALEZ, A; NICOVANI, S; MASSARDO, L; BULL, P; RODRIGUEZ, L; JACOBELLI, SObjective. The analysis of genetic markers of rheumatoid arthritis (RA) in a population in which the DR4 serotype is not strongly associated with the disease.
- ItemRADIOIMMUNOANALYSIS OF BLOOD CYCLOSPORINE CONCENTRATION IN KIDNEY-TRANSPLANT USING SOLID-PHASE SAMPLING(1987) FORADORI, AC; LOVELUCK, A; CORREA, L; RODRIGUEZ, L; MARTINEZ, L
- ItemTHE ARTERIOVENOUS OXYGEN GRADIENT AS AN INDEX OF RENAL BLOOD-FLOW - A STUDY IN DOG KIDNEYS AND RENAL-TRANSPLANT PATIENTS(ELSEVIER SCIENCE INC, 1992) MARTINEZ, L; TRONCOSO, P; COURT, F; DOUGNAC, A; RODRIGUEZ, L
- ItemWEAK ASSOCIATION BETWEEN HLA-DR4 AND RHEUMATOID-ARTHRITIS IN CHILEAN PATIENTS(BRITISH MED JOURNAL PUBL GROUP, 1990) MASSARDO, L; JACOBELLI, S; RODRIGUEZ, L; RIVERO, S; GONZALEZ, A; MARCHETTI, R