Browsing by Author "Pomes, Cristian"

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    Estetrol Increases Progesterone Genetic Response without Triggering Common Estrogenic Effects in Endometriotic Cell Lines and Primary Cultures
    (2023) Patino-Garcia, Daniel; Palomino, Jaime; Pomes, Cristian; Celle, Claudia; Torres-Estay, Veronica; Orellana, Renan
    Estetrol (E4), a natural estrogen produced by the human fetal liver, is actively studied for menopause and breast cancer treatment. It has low side effects and preferential estrogen receptor alpha (ER alpha) affinity. There are no data about its effects on endometriosis, a common gynecological disease affecting 6-10% of cycling women, generating painful pelvic lesions and infertility. Current combined hormone treatment (progestins and estrogens) is safe and efficient; nevertheless, one-third of patients develop progesterone (P4) resistance and recurrence by reducing P4 receptors (PRs) levels. We aimed to compare E4 and 17 beta-estradiol (E2) effects using two human endometriotic cell lines (epithelial 11Z and stromal Hs832 cells) and primary cultures from endometriotic patients. We evaluated cell growth (MTS), migration (wound assay), hormone receptors levels (Western blot), and P4 response by PCR array. Compared to E2, E4 did not affect cell growth or migration but increased estrogen receptor alpha (ER alpha) and PRs, and reduced ER beta. Finally, the incubation with E4 improved the P4 gene response. In conclusion, E4 increased PRs levels and genetic response without inducing cell growth or migration. These results suggest that E4 might be useful for endometriosis treatment avoiding P4 resistance; however, evaluating its response in more complex models is required.
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    Infantile/Capillary Hemangioma of the Uterine Corpus: A Rare Cause of Abnormal Genital Bleeding
    (2022) Ralph, Constanza; Cuello, Mauricio A.; Merino, Paulina M.; De Barbieri, Florencia M.; Astudillo, Katya; Huete, Alvaro; Garcia, Cristian; Pomes, Cristian
    Background: Infantile hemangiomas are vascular anomalies. However, they rarely cause genital bleeding. Here, we present the case of a young female with an endocavitary hemangioma who presented with abnormal uterine bleeding (AUB).Case: The patient was an 8-year-old female with genital bleeding. Transabdominal pelvic ultrasound showed a 20-mm highly vascularized focal intrauterine endocavitary lesion. Vascular computerized tomography excluded vascular anomalies. Magnetic resonance imaging sug-gested a hemangioma. Minimally invasive open surgery was performed to remove the lesion. Subsequent pathology analyses confirmed an infantile/capillary hemangioma. Conclusions: Infantile hemangiomas are vascular anomalies that should be considered potential causes of AUB in early puberty. The study of these cases should include pelvic ultrasound and vascular magnetic resonance imaging. Experienced surgeons can successfully accomplish fertility-sparing surgical procedures. Summary: We describe an unusual case of peripubertal AUB caused by an endocavitary capillary hemangioma. Management included fertility-sparing surgery and the complete resolution of symptoms.
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    Late postoperative urinary stress incontinence after a laparoscopic-assisted Vecchietti procedure for neovagina creation in a patient with Rokitansky syndrome: Successful treatment with the TVT-0 technique
    (ELSEVIER SCIENCE INC, 2007) Bianchi, Marcelo; Sola, Vicente; Ricci, Paolo; Pomes, Cristian
    A case report of urinary stress incontinence after surgery in a 20-year-old patient with Mayer-Rokitansky-Kuster-Hauser syndrome is presented. A neovagina was successfully created with a laparoscopic-assisted Vecchietti procedure. Fifteen months later, after normal and satisfactory sexual intercourse, the patient was seen for stress urinary incontinence. A second surgery consistent with the transobturator tension-free vaginal tape (TVT) system with nonelastic polypropylene suburethral TVT allowed full correction of the urinary symptoms. (C) 2007 AAGL. All rights reserved.