Browsing by Author "Palma, V."

Now showing 1 - 4 of 4
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    Independent Anti-Angiogenic Capacities of Coagulation Factors X and Xa
    (2014) Lange, S.; Gonzalez, I.; Pinto, M.; Arce, M.; Valenzuela Bassi, Rodrigo Andrés; Aranda, E.; Elliot, M.; Alvarez, M.; Henriquez, S.; Velasquez, Ever A.; Orge, F.; Oliva, B.; Gonzalez, P.; Villalón, Manuel J.; Cautivo Reyes, Kelly Margarita; Kalergis Parra, Alexis Mikes; Pereira, K.; Mendoza, C.; Saez, C.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Parborell, F.; Irusta, G.; Palma, V.; Allende, M.; Owen, Gareth Ivor
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    Platelet-Poor and Platelet-Rich Plasma Stimulate Bone Lineage Differentiation in Periodontal Ligament Stem Cells
    (2016) Martinez, C. E.; González Bombardiere, Sergio; Palma, V.; Smith, P. C.
    Background: Plasma-derived fractions have been used as an autologous source of growth factors; however, limited knowledge concerning their biologic effects has hampered their clinical application. In this study, the authors analyze the content and specific effect of both platelet-rich plasma (PRP) and platelet-poor plasma (PPP) on osteoblastic differentiation using primary cultures of human periodontal ligament stem cells (HPLSCs). Methods: The authors evaluated the growth factor content of PRP and PPP using a proteome profiler array and enzyme-linked immunosorbent assay. HPLSCs were characterized by flow cytometry and differentiation assays. The effect of PRP and PPP on HPLSC bone differentiation was analyzed by quantifying calcium deposition after 14 and 21 days of treatment. Results: Albeit at different concentrations, the two fractions had similar profiles of growth factors, the most representative being platelet-derived growth factor (PDGF) isoforms (PDGF-AA, -BB, and -AB), insulin-like growth factor binding protein (IGFBP)-2, and IGFBP-6. Both formulations exerted a comparable stimulus on osteoblastic differentiation even at low doses (2.5%), increasing calcium deposits in HPLSCs. Conclusions: PRP and PPP showed a similar protein profile and exerted comparable effects on bone differentiation. Further studies are needed to characterize and compare the effects of PPP and PRP on bone healing in vivo.
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    SHh activity and localization is regulated by perlecan
    (2011) Palma, V.; Carrasco Saavedra, Héctor Patricio; Larraín Correa, Juan Agustín
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    Sonic Hedgehog Stimulates Proliferation of Human Periodontal Ligament Stem Cells
    (SAGE PUBLICATIONS INC, 2011) Martinez, C.; Smith, P. C.; Rodriguez, J. P.; Palma, V.
    Regulation of cell renewal in the periodontium is a critical cell function that has not been clarified. Sonic hedgehog (Shh) is a secreted signaling molecule that plays a key role during development and adult tissue homeostasis. In the present study, we have analyzed the role played by Shh in human periodontal ligament stem cell (HPLSC) proliferation. HPLSC were isolated with anti-STRO-1 antibodies. Shh increased the expression of GLI1 and PTC-1 and selectively stimulated cell proliferation in STRO-1(+) derived from adult periodontal ligament. Shh components were localized to primary cilia in STRO-1(+) cells after Shh stimulation. STRO-1(+) also expressed Shh, suggesting an autocrine-regulated phenomenon. Thus, we propose that Shh plays a critical role in the regulation of cell proliferation in STRO-1(+)/HPLSC.