Browsing by Author "Osorio, Jose Miguel"

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    Interferon-β decreases LPS-induced neutrophil recruitment to cardiac fibroblasts
    (2023) Anfossi, Renatto; Vivar, Raul; Ayala, Pedro; Gonzalez-Herrera, Fabiola; Espinoza-Perez, Claudio; Osorio, Jose Miguel; Roman-Torres, Mauricio; Bolivar, Samir; Diaz-Araya, Guillermo
    Introduction: Cardiac fibroblasts (CF) are crucial cells in damaged heart tissues, expressing TLR4, IFN-receptor and responding to lipopolysaccharide (LPS) and interferon-beta (IFN-beta) respectively. While CF interact with immune cells; however, their relationship with neutrophils remains understudied. Additionally, theimpact of LPS and IFN-beta on CF-neutrophil interaction is poorly understood.Methods: Isolated CF from adult rats were treated with LPS, with or without IFN-beta. This study examined IL-8 secretion, ICAM-1 and VCAM-1 expression, and neutrophil recruitment, as well as their effects on MMPs activity.Results: LPS triggered increased IL-8 expression and secretion, along with elevated ICAM-1 and VCAM-1 expression, all of which were blocked by TAK-242. Pre-treatment with IFN-beta countered these LPS effects. LPS treated CF showed higher neutrophil recruitment (migration and adhesion) compared to unstimulated CF, an effect prevented by IFN-beta. Ruxolitinib blocked these IFN-beta anti-inflammatory effects, implicating JAK signaling. Analysis of culture medium zymograms from CF alone, and CF-neutrophils interaction, revealed that MMP2 was mainly originated from CF, while MMP9 could come from neutrophils. LPS and IFN-beta boosted MMP2 secretion by CF. MMP9 activity in CF was low, and LPS or IFN-beta had no significant impact. Pre-treating CF with LPS, IFN-beta, or both before co-culture with neutrophils increased MMP2. Neutrophil co-culture increased MMP9 activity, with IFN-beta pre-treatment reducing MMP9 compared to unstimulated CF.Conclusion: In CF, LPS induces the secretion of IL-8 favoring neutrophils recruitment and these effects were blocked by IFN-. The results highlight that CF-neutrophil interaction appears to influence the extracellular matrix through MMPs activity modulation.