Browsing by Author "Oses, Carolina"

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    Calcium (Ca2+) waves data calibration and analysis using image processing techniques
    (2013) Milovic Fabregat, Carlos Andrés; Oses, Carolina; Villalón, Manuel J.; Uribe Arancibia, Sergio A.; Prieto Vásquez, Claudia; Andía Kohnenkampf, Marcelo Edgardo; Irarrázaval Mena, Pablo; Tejos Núñez, Cristián Andrés; Lizama, Carlos
    Abstract Background Calcium (Ca2+) propagates within tissues serving as an important information carrier. In particular, cilia beat frequency in oviduct cells is partially regulated by Ca2+ changes. Thus, measuring the calcium density and characterizing the traveling wave plays a key role in understanding biological phenomena. However, current methods to measure propagation velocities and other wave characteristics involve several manual or time-consuming procedures. This limits the amount of information that can be extracted, and the statistical quality of the analysis. Results Our work provides a framework based on image processing procedures that enables a fast, automatic and robust characterization of data from two-filter fluorescence Ca2+ experiments. We calculate the mean velocity of the wave-front, and use theoretical models to extract meaningful parameters like wave amplitude, decay rate and time of excitation. Conclusions Measurements done by different operators showed a high degree of reproducibility. This framework is also extended to a single filter fluorescence experiments, allowing higher sampling rates, and thus an increased accuracy in velocity measurements.
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    Effect of Tamoxifen and Retinoic Acid on Bradykinin Induced Proliferation in MCF-7 Cells
    (2009) Paola Searovic; Alonso, Marcelo; Oses, Carolina; Pereira Flores, K.; Velarde Aliaga, María Victoria; Sáez Steeger, Claudia
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    Low physiological levels of prostaglandins E-2 and F-2 improve human sperm functions
    (2016) Rios, M.; Carreño Bustos, Daniela Verónica; Oses, Carolina; Barrera Rojas, Nelson Patricio; Kerr, B.; Villalón, Manuel J.
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    The Calcium Ion Is a Second Messenger in the Nitrate Signaling Pathway of Arabidopsis thaliana
    (2015) Riveras Hernández, Eleodoro Javier; Álvarez, José M.; Vidal, Elena A.; Oses, Carolina; Vega, Andrea; Gutiérrez Ilabaca, Rodrigo Antonio