Browsing by Author "Opazo, Juan C."
Now showing 1 - 7 of 7
Results Per Page
Sort Options
- ItemDiscovery of BbX transcription factor in the patagonian blennie: Exploring expression changes following combined bacterial and thermal stress exposure(2023) Martinez, Danixa; Nualart, Daniela; Loncoman, Carlos; Opazo, Juan C.; Zabala, Kattina; Morera, Francisco J.; Mardones, Gonzalo A.; Vargas-Chacoff, LuisHigh-Mobility Group (HMG) proteins are involved in different processes such as transcription, replication, DNA repair, and immune response. The role of HMG proteins in the immune response of fish has been studied mainly for HMGB1, where its expression can be induced by the stimulation of viral/bacterial PAMPs and can act as a proinflammatory mediator and as a global regulator of transcription in response to temperature. However, for BbX this role remains to be discovered. In this work, we identified the BbX of E. maclovinus and evaluated the temporal expression levels after simultaneous challenge with P. salmonis and thermal stress. Phylogenetic analysis does not significantly deviate from the expected organismal relationships suggesting orthologous relationships and that BbX was present in the common ancestor of the group. BbX mRNA expression levels were very high in the intestinal tissue of E. maclovinus (foregut, midgut, and hindgut). Nevertheless, the protein levels analyzed by WB showed the highest levels of BbX protein in the liver (constitutive expression). On the other hand, the mRNA expression levels of BbX in the liver of E. maclovinus injected with P. salmonis and subjected to thermal stress showed an increase at days 16 and 20 in all treatments applied at 12 degrees C and 18 degrees C. Meanwhile, the protein levels quantified by WB showed a statistically significant increase in the HMG-Bbx at all experimental times (4, 8, 12, 16, and 20 dpi). However, at 4 dpi the HMG-Bbx protein levels were much higher than the other days evaluated. The results suggest that BbX protein may be implicated in the response mechanism to temper-ature and bacterial stimulation in the foregut, midgut, hindgut, and liver, according to our findings at the level of mRNA and protein. Furthermore, our WB analysis suggests an effect of P. salmonis on the expression of this protein that can be observed in condition C+ 12 degrees C compared to C- 12 degrees C. Then, there is an effect of temperature that can be evidenced in the condition AM 18 degrees C and SM 18 degrees C, compared to AB 18 degrees C and SB 18 degrees C at 4, 8, and 12 dpi. We found not differences in the levels of this protein if the thermal stress is achieved through acclimatization or shock. More research is necessary to clarify the importance of this type of HMG in the immune response and thermal tolerance in fish.
- ItemEvolution of lysine-specific demethylase 1 and REST corepressor gene families and their molecular interaction(2023) Olivares-Costa, Montserrat; Oyarzun, Gianluca Merello; Verbel-Vergara, Daniel; Gonzalez, Marcela P.; Arancibia, Duxan; Andres, Maria E.; Opazo, Juan C.Lysine-specific demethylase 1A (LSD1) binds to the REST corepressor (RCOR) protein family of corepressors to erase transcriptionally active marks on histones. Functional diversity in these complexes depends on the type of RCOR included, which modulates the catalytic activity of the complex. Here, we studied the duplicative history of the RCOR and LSD gene families and analyzed the evolution of their interaction. We found that RCOR genes are the product of the two rounds of whole-genome duplications that occurred early in vertebrate evolution. In contrast, the origin of the LSD genes traces back before to the divergence of animals and plants. Using bioinformatics tools, we show that the RCOR and LSD1 interaction precedes the RCOR repertoire expansion that occurred in the last common ancestor of jawed vertebrates. Overall, we trace LSD1-RCOR complex evolution and propose that animal non-model species offer advantages in addressing questions about the molecular biology of this epigenetic complex.
- ItemEvolutionary history of the reprimo tumor suppressor gene family in vertebrates with a description of a new reprimo gene lineage(2016) Wichmann Pérez, Ignacio Alberto; Corvalán R., Alejandro; Amigo Donoso, Julio; Owen, Gareth Ivor; Zavala, Kattina.; Hoffmann, Federico G.; Vandewege, Michael W.; Opazo, Juan C.
- ItemExpression of RPRM/rprm in the olfactory system of embryonic zebrafish (Danio rerio)(2018) Stanic, Karen; Quiroz Vallverdu, Alonso Ingmar; Lemus, Carmen G.; Wichmann Pérez, Ignacio Alberto; Corvalán R., Alejandro; Owen, Gareth Ivor; Opazo, Juan C.; Concha, Miguel L.; Amigo Donoso, Julio
- ItemPhylogeography of the subterranean rodent Spalacopus cyanus (Caviomorpha, Octodontidae)(ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS, 2008) Opazo, Juan C.; Bugueno, Manuel P.; Carter, Mauricio J.; Palma, R. Eduardo; Bozinovic, FranciscoSpalacopus cyanus is a subterranean rodent inhabiting coastal and mountain habitats. Individuals from mountain populations are larger than individuals from the coast, and mountain populations have a more limited geographic range. To investigate the genetic structure and biogeography of this species, we analyzed mitochondrial DNA control region sequences. We found low levels of nucleotide diversity in comparison with other subterranean rodents. Coastal populations had higher nucleotide diversity and effective population size than mountain populations. Phylogenetic analysis using maximum parsimony and a haplotype network generated using statistical parsimony recognized 3 groups of haplotypes: northern coastal and mountain populations, central coastal populations, and southern coastal population. Consistent with the presence of unshared haplotypes, migration rates were practically 0, except from Valparaiso to Ventanas and from La Parva to Huentelauquen. We observed asymmetric migration rates from mountain to coastal populations, suggesting that this species originated in the Andean mountains. A likelihood ration test could not reject the null hypothesis of a stable population when all sequences were grouped into a single population and when coastal populations were analyzed separately. However, a negative exponential growth parameter was estimated for mountain populations,suggesting that these populations have undergone recent demographic changes.
- ItemReprimo tissue-specific expression pattern is conserved between zebrafish and human(2017) Figueroa, Ricardo J.; Wichmann Pérez, Ignacio Alberto; Owen, Gareth Ivor; Corvalán R., Alejandro; Amigo Donoso, Julio; Carrasco-Avino, Gonzalo.; Lange, Martin.; Siekmann, Arndt F.; Opazo, Juan C.
- ItemThe Reprimo Gene Family: A Novel Gene Lineage in Gastric Cancer with Tumor Suppressive Properties(2018) Amigo Donoso, Julio; Wichmann Pérez, Ignacio Alberto; Jorquera Cifuentes, Roddy Alberto.; García Bloj, Benjamín.; Alarcón Alarcón, María Alejandra; Owen, Gareth Ivor; Corvalán R., Alejandro; Opazo, Juan C.