Browsing by Author "Neira-Carrillo, Andronico"

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    Amino Acid-Functionalized Polyelectrolyte Films as Bioactive Surfaces for Cell Adhesion
    (2019) Leal, M. S.; Briones, X.; Villalobos, V.; Queneau, Y.; Leiva, A.; Rios, H. E.; Pavez, J.; Silva, C. P.; Carrasco, C.; Neira-Carrillo, Andronico; Roth, A. D.; Tamayo, L.; Urzua, M. D.
    Surfaces were prepared with polyelectrolyte derivatives of poly(styrene-alt-maleic anhydride) (PSMA) functionalized with amino acids of different hydropathy indices, with the aim of evaluating the effect of the chemical functionality of polyelectrolytes on SH-SY5Y neuroblastoma cell adhesion. Functionalizing PSMA derivatives with L-glutamine, L-methionine, and L-tyrosine yielded PSMA-Gln, PSMA-Met, and PSMA-Tyr polyelectrolytes, respectively. We first studied the adsorption behavior of PSMA functionalized with amino acids on silicon wafer surfaces modified with 3-aminopropyltriethoxysilane at pH 4.0 and 7.0 and at low and high ionic strengths. The highest rate of polyelectrolyte adsorption was at pH 4.0 and high ionic strength and was higher with the glutamine and tyrosine films. The advance contact angles (BA) of the polyelectrolyte surfaces showed a moderate effect of ionic strength and pH on polyelectrolyte film wettability, with PSMA-Tyr being slightly more hydrophobic. Atomic force microscopy images of the polyelectrolyte surfaces showed two types of morphology: the well-defined globular nanostructure of PSMA-Met and PSMA-Tyr and densely packed nanofibrous-like structure of PSMA-Gln. The highest level of ionic strength caused a slight decrease in the size of the nanostructure that formed the surface domains, which was reflected in the degree of surface roughness. Cell adhesion assays with the polyelectrolyte film showed that SH-SY5Y neuroblastoma cells cultured on PSMA-Met present a well-extended morphology characterized by a stellate shape, with five or more actin-rich thin processes, whereas SH-SY5Y cells that were seeded on PSMA-Gln and PSMAT-yr have a round morphology, with fewer and shorter processes. These results indicate that it is possible to modulate the surface characteristics of polyelectrolyte films based on their chemical functionality and environmental parameters such as pH and ionic strength in order to evaluate their effect on cell adhesion. Thus, surfaces prepared from polyelectrolytes functionalized with amino acids are an attractive and simple platform for cell adhesion, which can be used in developing biomaterials with modulated surface properties.
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    Blends based on amino acid functionalized poly (ethylene-alt-maleic anhydride) polyelectrolytes and PEO for nanofiber elaboration: Biocompatible and angiogenic polyelectrolytes
    (2022) Leal, Matias; Leiva, Angel; Villalobos, Valeria; Palma, Veronica; Carrillo, Daniela; Edwards, Natalie; Maine, Arianne; Cauich-Rodriguez, Juan, V; Tamayo, Laura; Neira-Carrillo, Andronico; Urzua, Marcela
    A wide variety of polymers have been electrospun to obtain nanofibers. However, obtaining nanofibers from polyelectrolytes is less frequent due to the charges of these polymers, which hinder the electrospinning process. Poly (ethylene-alt-maleic anhydride) (PEMA) was modified with a series of amino acids (Aa). The functionalization of PEMA with Aa (PEMA-Aa) was demonstrated by FT-IR, H-1 NMR, and C-13 NMR. Blends of PEMA-Aa and poly (ethylene oxide), PEO, with different ratios were prepared. Nanofibers were obtained by electrospinning using blends of 10-20% w/v of PEMA-Aa and 10% w/v of PEO. The conductivity of blends decreased, and the surface tension increased as the quantity of PEO in the blends was increased. TGA showed intermediate thermal properties compared with the blend components. Nanofibers were obtained for all PEMA-Aa/PEO blends, with diameters between 170 and 350 nm. Continuous fibers without morphological defects were obtained at concentrations of 20% w/v and 10% w/v of PEMA-Aa and PEO. Wharton's Jelly Mesenchymal Stem Cells viability, chicken embryo chorioallantoic membrane (CAM) assay and embryo viability measurements were realized for PEMA-Aa. Cytotoxicity test showed both composition and concentration-dependent behavior for PEMA-Aa, with higher WJ-MSC viability at 0.1 mg/mL at 24 h. CAM assay showed the formation of a high number of blood vessels and chicken embryo viability was close to 100% in the presence of polyelectrolytes. This, study demonstrates that electrospun nanofibers obtained from PEMA-Aa/PEO modified polyelectrolyte blends can be considered as a promising material for biomedical applications.