Browsing by Author "Musleh, Maher"

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    Novel Risk Associations between microRNA Polymorphisms and Gastric Cancer in a Chilean Population
    (2022) Landeros, Natalia; Corvalán R., Alejandro; Musleh, Maher; Quinones, Luis A.; Varela, Nelson M.; Gonzalez Hormazabal, Patricio
    Gastric cancer (GC) is the fifth leading cause of cancer deaths in the world, with variations across geographical regions and ethnicities. Emerging evidence indicates that miRNA expression is dysregulated in GC and its polymorphisms may contribute to these variations, which has yet to be explored in Latin American populations. In a case-control study of 310 GC patients and 311 healthy donors from Chile, we assessed the association of 279 polymorphisms in 242 miRNA genes. Two novel polymorphisms were found to be associated with GC: rs4822739:C>G (miR-548j) and rs701213:T>C (miR-4427). Additionally, rs1553867776:T>TCCCCA (miR-4274) and rs12416605:C>T (miR-938) were associated with intestinal-type GC, and rs4822739:C>G (miR-548j) and rs1439619:T>G (miR-3175) with TNM I-II stage. The polymorphisms rs6149511:T> TGAAGGGCTCCA (miR-6891), rs404337:G>A (miR-8084), and rs1439619:T>G (miR-3175) were identified among H.pylori-infected GC patients and rs7500280:T>C (miR-4719) and rs1439619:T>G (miR-3175) were found among H. pylori cagPAI+ infected GC cases. Prediction analysis suggests that seven polymorphisms could alter the secondary structure of the miRNA, and the other one is located in the seed region of miR-938. Targets of miRNAs are enriched in GC pathways, suggesting a possible biological effect. In this study, we identified seven novel associations and replicated one previously described in Caucasian population. These findings contribute to the understanding of miRNA genetic polymorphisms in the GC pathogenesis.
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    Prevalence of Helicobacter pylori Antimicrobial Resistance Among Chilean Patients
    (2021) Gonzalez-Hormazabal, Patricio; Arenas, Alex; Serrano, Carolina; Pizarro, Margarita; Fuentes-Lopez, Eduardo; Arnold, Jorge; Berger, Zoltan; Musleh, Maher; Valladares, Hector; Lanzarini, Enrique; Jara, Lilian; Castro, V. Gonzalo; Camargo, M. Constanza; Riquelme, Arnoldo
    Background. Treatments for Helicobacter pylori (H. pylori) eradication include the use of antibiotics and a proton-pump inhibitor. Antibiotic resistance is a major concern for two drugs: levofloxacin and clarithromycin. The aim was to determine the prevalence of levofloxacin resistance (LevoR) and clarithromycin resistance (ClaR) in an urban population in Santiago, Chile.