Browsing by Author "Moffett, James W. "
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- ItemCadmium-Copper Antagonism in Seaweeds Inhabiting Coastal Areas Affected by Copper Mine Waste Disposals(2006) Andrade, Santiago ; Medina, Matías H.; Moffett, James W. ; Correa M., Juan A.
- ItemKinetics of copper accumulation in Lessonia nigrescens (Phaeophyceae) under conditions of environmental oxidative stress(2006) Andrade, Santiago; Contreras, Loretto; Moffett, James W.; Correa, Juan A.Juvenile individuals of the brown kelp Lessonia nigrescens were exposed to a coastal environment chronically impacted by copper mine wastes and currently displaying more than 250 nM of total dissolved copper. The kinetic of copper accumulation in the intra and extracellular compartments was determined and correlated to the oxidative burst resulting from copper-mediated oxidative stress. Accumulation involved an initial adsorption onto the outer cell wall followed by a slower uptake into the cells. A linear pattern of copper uptake over time was found during the first 52 h of exposure, and a steady state was reached at 76 h. The resulting oxidative stress was found to be inefficiently attenuated, and the intracellular level of copper remained sufficiently high to determine a persistently higher than normal level of reactive oxygen species (ROS). Thus, our results strongly suggest that, in L. nigrescens, copper needs to reach an intracellular threshold before oxidative burst develops. Furthermore, it was found that the high ROS levels generated by copper accumulation within the cells persists after the oxidative burst has ceased, suggesting a limited capacity of the algal tissue to buffer the increases of ROS caused by the environmental copper levels. (c) 2006 Elsevier B.V. All rights reserved.
- ItemKinetics of Copper Accumulation in Lessonia Nigrescens (Phaeophyceae) Under Conditions of Environmental Oxidative Stress(2006) Andrade, Santiago ; Contreras, Loretto; Moffett, James W.; Correa M., Juan A.