Browsing by Author "Mills, David A."

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    A novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in Bifidobacterium longum subsp longum SC596
    (2016) Garrido, Daniel; Ruiz-Moyano, Santiago; Kirmiz, Nina; Davis, Jasmine C.; Totten, Sarah M.; Lemay, Danielle G.; Ugalde, Juan A.; German, J. Bruce; Lebrilla, Carlito B.; Mills, David A.
    The infant intestinal microbiota is often colonized by two subspecies of Bifidobacterium longum: subsp. infantis (B. infantis) and subsp. longum (B. longum). Competitive growth of B. infantis in the neonate intestine has been linked to the utilization of human milk oligosaccharides (HMO). However, little is known how B. longum consumes HMO. In this study, infant-borne B. longum strains exhibited varying HMO growth phenotypes. While all strains efficiently utilized lacto-N-tetraose, certain strains additionally metabolized fucosylated HMO. B. longum SC596 grew vigorously on HMO, and glycoprofiling revealed a preference for consumption of fucosylated HMO. Transcriptomes of SC596 during early-stage growth on HMO were more similar to growth on fucosyllactose, transiting later to a pattern similar to growth on neutral HMO. B. longum SC596 contains a novel gene cluster devoted to the utilization of fucosylated HMO, including genes for import of fucosylated molecules, fucose metabolism and two alpha-fucosidases. This cluster showed a modular induction during early growth on HMO and fucosyllactose. This work clarifies the genomic and physiological variation of infant-borne B. longum to HMO consumption, which resembles B. infantis. The capability to preferentially consume fucosylated HMO suggests a competitive advantage for these unique B. longum strains in the breast-fed infant gut.
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    Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria
    (2015) Garrido Cortés, Daniel; Ruiz-Moyano, Santiago; Lemay, Danielle G.; Sela, David A.; German, J. Bruce; Mills, David A.
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    Glycosylated proteins preserved over millennia : N-glycan analysis of Tyrolean Iceman, Scythian Princess and Warrior
    (2014) Ozcan, Sureyya; Kim, Bum Jin; Ro, Grace; Kim, Jae-Han; Bereuter, Thomas L.; Reiter, Christian; Dimapasoc, Lauren; Garrido Cortés, Daniel; Mills, David A.; Grimm, Rudolf; Lebrilla, Carlito B.; Joo An, Hyun
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    Hydrolysis of milk gangliosides by infant-gut associated bifidobacteria determined by microfluidic chips and high-resolution mass spectrometry
    (2014) Lee, Hyeyoung; Garrido, Daniel; Mills, David A.; Barile, Daniela
    Gangliosides are receiving considerable attention because they participate in diverse biological processes. Milk gangliosides appear to block pathogen adhesion and modify the intestinal ecology of newborns. However, the interaction of milk gangliosides with gut bifidobacteria has been little investigated. The digestion products of a mixture of gangliosides isolated from milk following incubation with six strains of bifidobacteria were studied using nanoHPLC Chip Q-TOF MS. To understand ganglioside catabolism in vitro, the two major milk gangliosidesGM3 and GD3remaining in the media after incubation with bifidobacteria were quantified. Individual gangliosides were identified through postprocessing precursor ion scans, and quantitated with the find by molecular feature algorithm of MassHunter Qualitative Analysis software. Bifidobacterium infantis and B. bifidum substantially degraded the GM3 and GD3, whereas B. longum subsp. longum and B. animalis subsp. lactis only showed moderate degradation. MALDI FTICR MS analysis enabled a deeper investigation of the degradation and identified ganglioside degradation specifically at the outer portions of the glycan molecules. These results indicate that certain infant gut-associated bifidobacteria have the ability to degrade milk gangliosides releasing sialic acid, and that these glycolipids could play a prebiotic role in the infant gut.
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    Identification of Oligosaccharides in Feces of Breast-fed Infants and Their Correlation with the Gut Microbial Community
    (2016) Jasmine, C. C.; Davis, Sarah M.; Totten, Julie O.; Huang, Sadaf Nagshbandi; Kirmiz, Nina; Garrido Cortés, Daniel; Lewis, Zachery T.; Wu, Lauren D.; Smilowitz, Jennifer T.; German, J. Bruce; Mills, David A.; Lebrilla, Carlito B.