Browsing by Author "Mardones, Pablo"

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    Advances in the physiological and pathological implications of cholesterol
    (2013) Cortés Mora, Víctor Antonio; Busso, Dolores; Mardones, Pablo; Maiz Gurruchaga, Manuel Alberto; Arteaga Ll., Antonio; Nervi, Flavio; Rigotti Rivera, Attilio
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    Impaired biliary cholesterol secretion and decreased gallstone formation in apolipoprotein E-deficient mice fed a high-cholesterol diet
    (2000) Amigo Boker, Ludwig Peter; Quiñones, Verónica; Mardones, Pablo; Zanlungo Matsuhiro, Silvana; Miquel Poblete, Juan Francisco; Nervi Oddone, Flavio; Rigotti Rivera, Attilio Gianpietro
    Background & aimsBecause apolipoprotein E (apoE) is a key cholesterol transport molecule involved in the hepatic uptake of chylomicron cholesterol, it may play a critical role in controlling bile cholesterol elimination and cholesterol gallstone formation induced by dietary cholesterol. To test this hypothesis, we studied biliary lipid secretion and gallstone formation in apoE-deficient mice fed cholesterol-rich diets.MethodsBile lipid outputs and gallstone sequence events were analyzed in apoE-deficient mice fed a high-cholesterol diet or a lithogenic diet compared with control animals.ResultsA high-cholesterol diet increased biliary cholesterol secretion and gallbladder bile cholesterol concentration in wild-type mice; the increase in bile cholesterol secretion was significantly attenuated in apoE-deficient mice. ApoE knockout mice fed a high-cholesterol lithogenic diet had a markedly lower frequency of gallbladder bile cholesterol crystal and gallstone formation than wild-type mice, which was most likely a result of the decreased cholesterol saturation index found in gallbladder bile of apoE-deficient mice.ConclusionsThese results show that apoE expression is an important factor for regulating both biliary secretion of diet-derived cholesterol as well as diet-induced cholesterol gallstone formation in mice.