Browsing by Author "Manubens, A"

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    Differential regulation of genes encoding manganese peroxidase (MnP) in the basidiomycete Ceriporiopsis subvermispora
    (2003) Manubens, A; Avila, M; Canessa, P; Vicuña, R
    We previously identified and characterized three mnp genes coding for manganese peroxidase (MnP) in the white rot fungus Ceriporiopsis subvermispora. In this work, we assessed transcript levels of mnp genes in liquid cultures of this fungus grown under various conditions. In the absence of Mn2+, mnp1 and mnp2 mRNA were detected by Northern hybridization, irrespective of the lack of extracellular MnP activity. Addition of Mn2+ to the cultures led to a marked increase in both transcripts, the highest titers being observed at 10 muM Mn2+ mnp1 mRNA was not detected at Mn2+ concentrations above 80 muM, whereas mnp2 mRNA was still observed at 320 muM Mn2+. Differential regulation of these genes was confirmed by the addition of Cu2+, Zn2+, Ag+ and Cd2+. These metal ions dramatically elevated both transcripts and also allowed the detection of the mnp3 transcript. In most cases, the increase in mRNA levels was partially abolished by the simultaneous presence of Mn2+, although the latter was strictly required to detect extracellular MnP activity. However, the lignin-related compound syringic acid specifically increased the mnp1 transcript, although only in the absence of Mn2+. These results indicate that there is no clear correlation between mnp mRNA levels and MnP activity. In addition, they strongly suggest that Mn2+ plays a post-transcriptional role which is essential for the presence of active MnP in the extracellular fluid.
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    Enzymology and molecular genetics of the ligninolytic system of the basidiomycete Ceriporiopsis subvermispora
    (2001) Lobos, S; Tello, M; Polanco, R; Larrondo, LF; Manubens, A; Salas, L; Vicuña, R
    Lignin, the most abundant renewable source of aromatic carbon on earth, consists in a highly irregular three-dimensional biopolymer of oxygenated phenylpropanoid units. In natural environments, lignin is only degraded efficiently by some fungi belonging to the group of basidiomycetes. These microorganisms secrete an array of oxidases and peroxidases for this purpose, which may be produced in various combinations. This article summarizes our studies on a particular strain called Ceriporiopsis subvermispora, a fungus which is highly agressive towards lignin when growing on wood.