Browsing by Author "Leng, Yaozhu"

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    Are B cells altered in the decidua of women with preterm or term labor?
    (2019) Leng, Yaozhu; Romero, Roberto; Xu, Yi; Galaz Alarcón, José; Slutsky, Rebecca; Arenas Hernández, Marcia; García Flores, Valeria; Motomura, Kenichiro; Hassan, Sonia S.; Reboldi, Andrea; Gómez López, Nardhy
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    Distinct Cellular Immune Responses to SARS-CoV-2 in Pregnant Women
    (2022) Gomez-Lopez, Nardhy ; Romero, Roberto ; Tao, Li ; Gershater, Meyer ; Leng, Yaozhu ; Zou, Chengrui; Farias-Jofre, Marcelo ; Galaz, Jose ; Miller, Derek; Tarca, Adi L. ; Arenas-Hernandez, Marcia ; Bhatti, Gaurav; Garcia-Flores, Valeria ; Liu, Zhenjie ; Para, Robert ; Kanninen, Tomi ; Hadaya, Ola ; Paredes, Carmen ; Xu, Yi
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    Exhausted and Senescent T Cells at the Maternal-Fetal Interface in Preterm and Term Labor
    (2019) Slutsky, Rebecca; Romero, Roberto; Xu, Yi; Galaz, Jose; Miller, Derek; Done, Bogdan; Tarca, Adi L.; Gregor, Sabrina; Hassan, Sonia S.; Leng, Yaozhu; Gomez-Lopez, Nardhy
    Successful pregnancy requires a tightly-regulated equilibrium of immune cell interactions at the maternal-fetal interface (i.e., the decidual tissues), which plays a central role in the inflammatory process of labor. Most of the innate immune cells in this compartment have been well characterized; however, adaptive immune cells are still under investigation. Herein, we performed immunophenotyping of the decidua basalis and decidua parietalis to determine whether exhausted and senescent T cells are present at the maternal-fetal interface and whether the presence of pathological (i.e., preterm) or physiological (i.e., term) labor and/or placental inflammation alter such adaptive immune cells. In addition, decidual exhausted T cells were sorted to test their functional status. We found that (1) exhausted and senescent T cells were present at the maternal-fetal interface and predominantly expressed an effector memory phenotype, (2) exhausted CD4(+) T cells increased in the decidua parietalis as gestational age progressed, (3) exhausted CD4(+) and CD8(+) T cells decreased in the decidua basalis of women who underwent labor at term compared to those without labor, (4) exhausted CD4(+) T cells declined with the presence of placental inflammation in the decidua basalis of women with preterm labor, (5) exhausted CD8(+) T cells decreased with the presence of placental inflammation in the decidua basalis of women who underwent labor at term, (6) both senescent CD4(+) and CD8(+) T cells declined with the presence of placental inflammation in the decidua basalis of women who underwent preterm labor, and (7) decidual exhausted T cells produced IFN and TNF upon in vitro stimulation. Collectively, these findings indicate that exhausted and senescent T cells are present at the human maternal-fetal interface and undergo alterations in a subset of women either with labor at term or preterm labor and placental inflammation. Importantly, decidual T cell function can be restored upon stimulation.
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    The immunophenotype of amniotic fluid leukocytes in normal and complicated pregnancies
    (WILEY, 2018) Gomez Lopez, Nardhy; Romero, Roberto; Xu, Yi; Miller, Derek; Leng, Yaozhu; Panaitescu, Bogdan; Silva, Pablo; Faro, Jonathan; Alhousseini, Ali; Gill, Navleen; Hassan, Sonia S.; Hsu, Chaur Dong
    ProblemThe immune cellular composition of amniotic fluid is poorly understood. Herein, we determined: 1) the immunophenotype of amniotic fluid immune cells during the second and third trimester in the absence of intra-amniotic infection/inflammation; 2) whether amniotic fluid T cells and ILCs display different phenotypical characteristics to that of peripheral cells; and 3) whether the amniotic fluid immune cells are altered in women with intra-amniotic infection/inflammation.