Browsing by Author "Leiva-Aravena, Enzo"

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    Arsenic removal mediated by acidic ph neutralization and iron precipitation in microbial fuel cells
    (2018) Leiva, Eduardo; Leiva-Aravena, Enzo; Rodriguez, Carolina; Serrano, Jennyfer; Vargas Cucurella, Ignacio Tomás; CEDEUS (Chile)
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    Benefits of a Recombination-Proficient Escherichia coli System for Adaptive Laboratory Evolution
    (2016) Peabody, George; Winkler, James; Fountain, Weston; Castro, David A.; Leiva-Aravena, Enzo; Kao, Katy C.
    Adaptive laboratory evolution typically involves the propagation of organisms asexually to select for mutants with the desired phenotypes. However, asexual evolution is prone to competition among beneficial mutations (clonal interference) and the accumulation of hitchhiking and neutral mutations. The benefits of horizontal gene transfer toward overcoming these known disadvantages of asexual evolution were characterized in a strain of Escherichia coli engineered for superior sexual recombination (genderless). Specifically, we experimentally validated the capacity of the genderless strain to reduce the mutational load and recombine beneficial mutations. We also confirmed that inclusion of multiple origins of transfer influences both the frequency of genetic exchange throughout the chromosome and the linkage of donor DNA. We built a simple kinetic model to estimate recombination frequency as a function of transfer size and relative genotype enrichment in batch transfers; the model output correlated well with the experimental data. Our results provide strong support for the advantages of utilizing the genderless strain over its asexual counterpart during adaptive laboratory evolution for generating beneficial mutants with reduced mutational load.