Browsing by Author "Leighton Puga, Federico"

Now showing 1 - 6 of 6
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    Complementary effects of Mediterranean diet and moderate red wine intake on haemostatic cardiovascular risk factors
    (NATURE PUBLISHING GROUP, 2001) Mezzano, Diego; Leighton Puga, Federico; Martínez, Carlos; Marshall Rivera, Guillermo; Cuevas Marín, Ada Marisa; Castillo Valenzuela, Oscar; Panes Becerra, Olga Teresa; Rozowski Narkunska, Samuel Jaime; Pérez Pons, Druso Diego; Mizón Costa, Claudio Luis Enrique; San Martin, Alejandra; Pereira Pereira, J. Marcello
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    Diagnosis of mild platelet function disorders. Reliability and usefulness of light transmission platelet aggregation and serotonin secretion assays
    (2009) Quiroga Gutiérrez, Sara Teresita; Goycolea, Manuela; Matus Ritter, Valeria; Zúñiga Contreras, Pamela; Martínez, Carlos; Garrido, Marcelo; Aranda Lauriani, Eduardo Javier; Leighton Puga, Federico; Panes Becerra, Olga Teresa; Pereira Garcés, Jaime Ignacio; Mezzano, Diego
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    Effect of Mediterranean and occidental diets, and red wine, on plasma fatty acids in humans. An intervention study
    (2004) Urquiaga Reus, Inés; Guasch Castro, Viviana; Marshall Rivera, Guillermo; San Martin, Alejandra; Castillo Valenzuela, Oscar; Rozowski Narkunska, Samuel Jaime; Leighton Puga, Federico
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    Lipoperoxidation and protein oxidative damage exhibit different kinetics during septic shock
    (HINDAWI LTD, 2008) Andresen Hernández, Max; Regueira Heskia, Tomás; Bruhn, Alejandro; Pérez Pons, Druso Diego; Strobel Lobos, Pablo Alberto; Dougnac Labatut, Alberto; Marshall Rivera, Guillermo; Leighton Puga, Federico
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    Lysosomal vitamin E accumulation in Niemann-Pick type C disease
    (2011) Yévenes, Luz Fernanda; Klein Posternack, Andrés David; Castro, Juan Francisco; Marín Marín, Tamara Alejandra; Leal Reyes, Nancy Valeria; Leighton Puga, Federico; Álvarez, Alejandra R.; Zanlungo Matsuhiro, Silvana
    Niemann-Pick C disease (NPC) is a neuro-visceral lysosomal storage disorder mainly caused by genetic defects in the NPC1 gene. As a result of loss of NPC1 function large quantities of free cholesterol and other lipids accumulate within late endosomes and lysosomes. In NPC livers and brains, the buildup of lipids correlates with oxidative damage; however the molecular mechanisms that trigger it remain unknown. Here we study potential alterations in vitamin E (α-tocopherol, α-TOH), the most potent endogenous antioxidant, in liver tissue and neurons from NPC1 mice. We found increased levels of α-TOH in NPC cells. We observed accumulation and entrapment of α-TOH in NPC neurons, mainly in the late endocytic pathway. Accordingly, α-TOH levels were increased in cerebellum of NPC1 mice. Also, we found decreased mRNA levels of the α-TOH transporter, α-Tocopherol Transfer Protein (α-TTP), in the cerebellum of NPC1 mice. Finally, by subcellular fractionation studies we detected a significant increase in the hepatic α-TOH content in purified lysosomes from NPC1 mice. In conclusion, these results suggest that NPC cells cannot transport vitamin E correctly leading to α-TOH buildup in the endosomal/lysosomal system. This may result in a decreased bioavailability and impaired antioxidant function of vitamin E in NPC, contributing to the disease pathogenesis.
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    Mediterranean diet and red wine protect against oxidative damage in young volunteers
    (ELSEVIER IRELAND LTD, 2010) Urquiaga Reus, Inés; Strobel Lobos, Pablo Alberto; Pérez Pons, Druso Diego; Martinez, Carlos; Cuevas Marin, Ada Marisa; Castillo Valenzuela, Oscar; Marshall Rivera, Guillermo; Rozowski Narkunska, Samuel Jaime; Leighton Puga, Federico