Browsing by Author "Le-Feuvre, Regis"

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    Biotechnology applied toHaematococcus pluvialisFotow: challenges and prospects for the enhancement of astaxanthin accumulation
    (2020) Le-Feuvre, Regis; Moraga-Suazo, Priscila; Gonzalez, Javiera; Martin, Sergio San; Henriquez, Vitalia; Donoso, Andrea; Agurto-Munoz, Cristian
    Among natural sources, the green algaHaematococcus pluvialisis the major producer of the potent antioxidant pigment astaxanthin, a high-value compound whose demand is still not sufficiently covered by the current industrial production. Despite the availability of low-cost synthetic astaxanthin, natural astaxanthin is more potent and accepted for human consumption and food additive uses. This review highlights the use of different biotechnological approaches aiming to increase astaxanthin production yields and discusses the advantages and drawbacks of traditional alternatives widely used on other microorganisms. These traditional approaches span from the easy to perform but not devoid of problems random mutagenesis, to advanced methods like microalgae genetic engineering, which has great potential for enhancement, despite being highly restricted in several countries by genetically modified organism legislation. In addition, we propose the underexplored approach of artificial polyploidization for the obtention of strains with increased cell size, which have the advantage of being considered as non-genetically modified organisms that do not require modification of the current industrial production procedures.
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    Relative Expression of Genes Elicited by Clonostachys rosea in Pinus radiata Induces Systemic Resistance
    (2024) Moraga-Suazo, Priscila; Le-Feuvre, Regis; Navarrete, Dario; Sanfuentes, Eugenio
    Radiata pine is one of the most commonly planted tree species in Chile due to its fast growth and desirable wood and pulp properties. However, its productivity is hampered by several diseases. Pitch canker disease (PCC) caused by Fusarium circinatum, is considered the most damaging disease to the pine forest industry. Several control measures have been established, with biological control emerging as an environmentally friendly and effective way for F. circinatum control. Previous studies support the value of Clonostachys rosea in reducing PCC damage, with evidence suggesting a potential induced systemic resistance (ISR) triggered in radiata pines by this agent. Ten-month-old radiata pine plants were pre-treated with C. rosea on a substrate at 8 and 1 days before inoculation with F. circinatum on the stem tip, and expression levels were determined for DXS1, LOX, PAL, and PR3 genes 24 h later. Lesion length was 45% lower on plants pre-treated with C. rosea and infected with F. circinatum compared to non-pre-treated and infected plants. Additionally, LOX and PR3 were induced 23 and 62 times more, respectively, in comparison to untreated plants. Our results indicate that C. rosea causes an ISR response in pre-treated plants, significantly increasing the expression of resistance genes and reducing lesion length.