Browsing by Author "Larrondo Castro, Luis Fernando"
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- ItemA circadian clock in Neurospora crassa functions during plant cell wall deconstruction(2020) Diaz, R. D.; Larrondo Castro, Luis Fernando
- ItemA novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity(2003) Larrondo Castro, Luis Fernando; Salas, L.; Melo Ledermann, Francisco Javier; Vicuña, Rafael; Cullen, D.Lignin degradation by the white rot basidiomycete Phanerochaete chrysosporium involves various extracellular oxidative enzymes, including lignin peroxidase, manganese peroxidase, and a peroxide-generating enzyme, glyoxal oxidase. Recent studies have suggested that laccases also may be produced by this fungus, but these conclusions have been controversial. We identified four sequences related to laccases and ferroxidases (Fet3) in a search of the publicly available P. chrysosporium database. One gene, designated mco1, has a typical eukaryotic secretion signal and is transcribed in defined media and in colonized wood. Structural analysis and multiple alignments identified residues common to laccase and Fet3 sequences. A recombinant MCO1 (rMCO1) protein expressed in Aspergillus nidulans had a molecular mass of 78 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the copper I-type center was confirmed by the UV-visible spectrum. rMCO1 oxidized various compounds, including 2,2′-azino(bis-3-ethylbenzthiazoline-6-sulfonate) (ABTS) and aromatic amines, although phenolic compounds were poor substrates. The best substrate was Fe2+, with a Km close to 2 μM. Collectively, these results suggest that the P. chrysosporium genome does not encode a typical laccase but rather encodes a unique extracellular multicopper oxidase with strong ferroxidase activity.
- ItemDaily magnesium fluxes regulate cellular timekeeping and energy balance(2016) Kevin, A.; Feeney, Louise L.; Hansen, Marrit Putker; Olivares Yañez, Consuelo; Day, Jason; Eades, Lorna J.; Larrondo Castro, Luis Fernando; Nathaniel, P.; Hoyle, John S; O'Neill; Gerben Van Ooijen
- ItemFunctional analysis of the endoxylanase B (xynB) promoter from Penicillium purpurogenum(2008) Díaz Muñoz, Jheimmy Maríana; Larrondo Castro, Luis Fernando; Eyzaguirre, Jaime; Bull Simpfendorfer, Paulina
- ItemIndirect monitoring of TORC1 signalling pathway reveals molecular diversity among different yeast strains(2019) Kessi‐Pérez, Eduardo I.; Salinas, Francisco; Molinet, Jennifer; González, Asier; Muñiz, Sara; Guillamón, José M.; Hall, Michael N.; Larrondo Castro, Luis Fernando; Martínez, Claudio
- ItemRIM15 antagonistic pleiotropy is responsible for differences in fermentation and stress response kinetics in budding yeast(2016) Kessi Pérez, Eduardo I.; Araos, Sebastián; García, Verónica; Salinas, Francisco; Abarca, Valentina; Larrondo Castro, Luis Fernando; Martínez, Claudio; Cubillos, Francisco A.
- ItemThe developmental regulator Pcz1 affects the production of secondary metabolites in the filamentous fungus Penicillium roqueforti(2018) Rojas Aedo, Juan F.; Gil Durán, Carlos; Goity Falconi, Alejandra Isabel; Vaca, Inmaculada; Levicán, Gloria; Larrondo Castro, Luis Fernando; Chávez, Renato