Browsing by Author "Labayen, Idoia"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Association between GIP levels after glucose load and HMW adiponectin in normoglycemic women
    (2025) Salvatierra Matzner, Isidora; Parada, Javier; Cataldo Bascuñan, Luis Rodrigo; Galgani Fuentes, José; Alberti, Gigliola; Labayen, Idoia; Lorenzoni Santos, José Guillermo
    Background/Objectives: Glucose-dependent insulinotropic polypeptide (GIP) is secreted by enteroendocrine K cells in response to nutrient ingestion. The aims of this study are: 1) to evaluate the cross-sectional associations between plasma GIP change in response to an oral glucose challenge (as a surrogate of GIP secretion) with obesity-related anthropometric measurements, fasting inflammatory biomarkers, and fasting circulating adipokines. 2) to evaluate the feasibility of using postprandial plasma GIP as a biomarker of adiposity-related phenotypes in response to starchbased meals. Methods: Fifty normoglycemic women without obesity (19-32 years) were evaluated with an oral glucose tolerance test (OGTT). A feasibility study was conducted in a subset of eight women to estimate responses to starch-based meals (25 g of starch). Postprandial glycemic-related changes in plasma hormones/metabolites were assessed, as well as circulating adipokines and inflammatory biomarkers in fasting conditions. Results: The Incremental-GIP change after 2-hour OGTT was significantly associated with waist circumference (rho=0.34; P=0.02), fasting plasma TNFα (rho=0.54; P=0.0002), and white blood cell count (rho=0.39; P=0.008), but not with MCP-1, total adiponectin, leptin, or the free leptin index. A strong inverse association was found between incremental-GIP change and fasting plasma High-Molecular-Weight (HMW) Adiponectin (rho = -0.50; P = 0.0004), which remained significant after adjusting for age and body mass index. Conclusion: An inverse association was found between postprandial GIP levels and circulating HMW-adiponectin levels in humans. This research highlights the suitability of using postprandial plasma GIP as a biomarker for metabolic disturbances of increased adiposity, even in the absence of obesity.
  • No Thumbnail Available
    Item
    Deciphering the constrained total energy expenditure model in humans by associating accelerometer-measured physical activity from wrist and hip
    (2021) Fernandez-Verdejo, Rodrigo; Alcantara, Juan M. A.; Galgani, Jose E.; Acosta, Francisco M.; Migueles, Jairo H.; Amaro-Gahete, Francisco J.; Labayen, Idoia; Ortega, Francisco B.; Ruiz, Jonatan R.
    The constrained total energy expenditure (TEE) model posits that progressive increases in physical activity (PA) lead to increases in TEE; but after certain PA threshold, TEE plateaus. Then, a compensatory reduction in the expenditure of non-essential activities constrains the TEE. We hypothesized that high PA levels as locomotion associate with a compensatory attenuation in arm movements. We included 209 adults (64% females, mean [SD] age 32.1 [15.0] years) and 105 children (40% females, age 10.0 [1.1] years). Subjects wore, simultaneously, one accelerometer in the non-dominant wrist and another in the hip for >= 4 days. We analyzed the association between wrist-measured (arm movements plus locomotion) and hip-measured PA (locomotion). We also analyzed how the capacity to dissociate arm movements from locomotion influences total PA. In adults, the association between wrist-measured and hip-measured PA was better described by a quadratic than a linear model (Quadratic-R-2=0.54 vs. Linear-R-2=0.52; P=0.003). Above the 80th percentile of hip-measured PA, wrist-measured PA plateaued. In children, there was no evidence that a quadratic model fitted the association between wrist-measured and hip-measured PA better than a linear model (R-2=0.58 in both models, P=0.25). In adults and children, those with the highest capacity to dissociate arm movements from locomotion-i.e. higher arm movements for a given locomotion-reached the highest total PA. We conclude that, in adults, elevated locomotion associates with a compensatory reduction in arm movements (probably non-essential fidgeting) that partially explains the constrained TEE model. Subjects with the lowest arm compensation reach the highest total PA.
  • Thumbnail Image
    Item
    Reproducibility of the energy metabolism response to an oral glucose tolerance test: influence of a postcalorimetric correction procedure
    (2022) Alcantara, Juan M. A.; Sanchez-Delgado, Guillermo; Jurado-Fasoli, Lucas; Galgani Fuentes, José; Labayen, Idoia; Ruiz, Jonatan R.
    Metabolic flexibility (MetF), which is a surrogate of metabolic health, can be assessed by the change in the respiratory exchange ratio (RER) in response to an oral glucose tolerance test (OGTT). We aimed to determine the day-to-day reproducibility of the energy expenditure (EE) and RER response to an OGTT, and whether a simulation-based postcalorimetric correction of metabolic cart readouts improves day-to-day reproducibility. The EE was assessed (12 young adults, 6 women, 27 +/- 2 years old) using an Omnical metabolic cart (Maastricht Instruments, Maastricht, The Netherlands) after an overnight fast (12 h) and after a 75-g oral glucose dose on 2 separate days (48 h). On both days, we assessed EE in 7 periods (one 30-min baseline and six 15-min postprandial). The ICcE was performed immediately after each recording period, and capillary glucose concentration (using a digital glucometer) was determined. We observed a high day-to-day reproducibility for the assessed RER (coefficients of variation [CV] <4%) and EE (CVs < 9%) in the 7 different periods. In contrast, the RER and EE areas under the curve showed a low day-to-day reproducibility (CV = 22% and 56%, respectively). Contrary to our expectations, the postcalorimetric correction procedure did not influence the day-to-day reproducibility of the energy metabolism response, possibly because the Omnical's accuracy was similar to 100%. Our study demonstrates that the energy metabolism response to an OGTT is poorly reproducible (CVs >20%) even using a very accurate metabolic cart. Furthermore, the postcalorimetric correction procedure did not influence the day-to-day reproducibility.