Browsing by Author "KALTWASSER, G"
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- ItemBIOSYNTHESIS OF THE NEUROFILAMENT HEAVY SUBUNIT IN XENOPUS OOCYTES MICROINJECTED WITH RAT-BRAIN POLY(A)+ RNA(1987) CROSS, D; ALLENDE, ML; KRAUSS, RY; FUENTES, ME; KALTWASSER, G; ALVAREZ, J; INESTROSA, NCIn order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.
- ItemENZYMATIC DNA AMPLIFICATION (PCR) IN THE DIAGNOSIS OF EXTRAPULMONARY MYCOBACTERIUM-TUBERCULOSIS INFECTION(ACADEMIC PRESS LTD, 1993) KALTWASSER, G; GARCIA, S; SALINAS, AM; MONTIEL, F
- ItemINVITRO ANTIBACTERIAL ACTIVITY OF TROSPECTOMYCIN (U-63,366F) AGAINST ANAEROBIC-BACTERIA AND AEROBIC GRAM-POSITIVE COCCI IN CHILE(ELSEVIER SCIENCE INC, 1991) MONTIEL, F; KALTWASSER, G; PINTO, ME; LAM, MThe in vitro activity of trospectomycin sulfate was compared with those of several antimicrobials, against 301 anaerobic bacteria and 613 aerobic Gram-positive cocci. Trospectomycin was about 4- to 32-fold more active than was spectinomycin. Trospectomycin exhibited consistently good activity against all Bacteroides fragilis group isolates, except Bacteroides vulgatus, and against all other anaerobes comparable or higher to that of clindamycin. The trospectomycin's activity was most similar to that of vancomycin, even against methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis.
- ItemINVITRO SUSCEPTIBILITY TO 10 ANTIBIOTICS OF METHICILLIN-RESISTANT (MRSA) STAPHYLOCOCCUS-AUREUS IN CHILE(1988) MONTIEL, F; KALTWASSER, G; VALDIVIESO, C; LAM, MThe in vitro activity of 10 antibiotics was determined for 231 strains of methicillin resistant (MRSA) Staphylococcus aureus. Oxacillin was a very good antibiotic to determine methicillin-resistance. Its agreement with methicillin-resistance was in all the strains tested. On the contrary, the correlation with nafcillin was established only in 95% of the strains tested. Cloxacillin and flucloxacillin are not good methicillin-resistance indicators. The strains tested against macrolides, such as erythromycin, and lincosamides, such as lincomycin and clindamycin presented a susceptibility of 68, 78, and 80%, respectively. All tested strains were susceptible to vancomycin.