Browsing by Author "JOYCE, TW"

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    DEGRADATION OF 4,5-DICHLOROGUAIACOL BY SOIL-MICROORGANISMS
    (1995) GONZALEZ, B; BREZNY, R; HERRERA, M; JOYCE, TW
    No microorganisms could be isolated from chemostats or from a soil column fed with 4,5-dichloroguaiacol as the only carbon source. If guaiacol was added to chemostats with 4,5-dichloroguaiacol, either soil microbial consortia or guaiacol-degrading bacteria could dechlorinate the 4,5-dichloroguaiacol provided it was < 0.2 mM. A microbial consortium from farm soil removed 4,5-dichloroguaiacol under aerobic or anoxic conditions, with or without chlorolignin. Dichlorocatechol was the only 4,5-dichloroguaiacol-derived metabolite detected. In aerobic incubations, 4,5-dichlorocatechol was further degraded whereas under anoxic conditions it accumulated.
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    METABOLISM OF MONOCHLORINATED AND DICHLORINATED GUAIACOLS BY RHODOCOCCUS RUBER CA16
    (1995) ACEVEDO, C; BREZNY, R; JOYCE, TW; GONZALEZ, B
    The metabolism of chloroguaiacols by a soil bacterium was studied. The strain was isolated by enrichment, with guaiacol as the sole carbon and energy source, and identified as a Rhodococcus ruber CA16. None of seven chlorinated guaiacols supported bacterial growth. However, ultraviolet spectroscopy, chloride release, and oxygen consumption showed that resting cells grown on guaiacol degraded completely 4-chloroguaiacol, 5-chloroguaiacol, and 6-chloroguaiacol and, to a lesser extent, 4,5-dichloroguaiacol. Gas chromatographic analysis suggested microbial formation of 4-chlorocatechol and 4,5-dichlorocatechol from 4-chloroguaiacol and 4,5-dichloroguaiacol, respectively. Although mono- and dichloroguaiacols did not affect the strain's ability to grow on guaiacol, chlorocatechols completely arrested growth. The role of chlorocatechols in chloroguaiacol metabolism by this guaiacol-degrading bacterial strain is discussed.