Browsing by Author "Inostroza Blancheteau, Claudio"

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    Improved Salinity Tolerance in Carrizo Citrange Rootstock through Overexpression of Glyoxalase System Genes
    (2015) Alvarez Gerding, Ximena; Cortés Bullemore, Rowena; Medina, Consuelo; Romero Romero, Jesús L.; Inostroza Blancheteau, Claudio; Aquea Zeballos, José Felipe; Arce Johnson, Jorge Patricio
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    Isolation and molecular characterization of MYB60 in Solanum lycopersicum
    (2021) Rodríguez Hoces de la Guardia, Amparo Cecilia; Ugalde, María Beatriz; Lobos Díaz, Valeria Rocío; Lucina Romero-Romero, Jesús; Meyer Regueiro, Carlos José; Inostroza Blancheteau, Claudio; Reyes Diaz, Marjorie; Aquea, Felipe; Arce Johnson, Jorge Patricio
    Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.
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    Short-term UV-B radiation affects photosynthetic performance and antioxidant gene expression in highbush blueberry leaves
    (2016) Inostroza Blancheteau, Claudio; Acevedo, Patricio; Loyola, Rodrigo; Arce Johnson, Jorge Patricio; Alberdi, Miren; Reyes Díaz, Marjorie
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    Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis
    (2018) Romero Romero, Jesús L.; Inostroza Blancheteau, Claudio; Orellana, Daniela; Aquea Zeballos, José Felipe; Reyes Díaz, Marjorie; Gil Montenegro, Pilar Macarena; Matte Risopatrón, Juan Pablo; Arce Johnson, Jorge Patricio