Browsing by Author "HINRICHSEN, V"

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    ENTEROPATHOGENICITY OF AEROMONAS SPECIES ISOLATED FROM INFANTS - A COHORT STUDY
    (1988) FIGUEROA, G; GALENO, H; SOTO, V; TRONCOSO, M; HINRICHSEN, V; YUDELEVICH, A
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    ROTAVIRUS DETECTION BY DOT BLOT HYBRIDIZATION ASSAY USING A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE
    (1992) FERNANDEZ, J; SANDINO, A; YUDELEVICH, A; AVENDANO, LF; VENEGAS, A; HINRICHSEN, V; SPENCER, E
    A synthetic oligodeoxynucleotide of 40 nucleotides corresponding to nucleotides 33-72 of the gene coding for the viral protein VP7 of rotavirus. was used as a nucleic acid probe to develop a non-radioactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.