Browsing by Author "Gutierrez-Cutino, Marlen"

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    A new strategy to build electrochemical enzymatic biosensors using a nanohybrid material based on carbon nanotubes and a rationally designed schiff base containing boronic acid
    (2024) Tamborelli, Alejandro; Mujica, Michael Lopez; Sanchez-Velasco, Oriel A.; Hormazabal-Campos, Cristobal; Perez, Edwin G.; Gutierrez-Cutino, Marlen; Venegas-Yazigi, Diego; Dalmasso, Pablo; Rivas, Gustavo; Hermosilla-Ibanez, Patricio
    We report a nanohybrid material obtained by non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with the new ligand (((1E,1 ' E)-(naphthalene-2,3-diylbis(azaneylylidene))bis(methaneylylidenedene)) bis (4-hyoxy-3,1-phenylene))doronic acid (SB-dBA), rationally designed to mimic some recognition properties of biomolecules like concanavalin A, for the development of electrochemical biosensors based on the use of glycobiomolecules as biorecognition element. We present, as a proof-of-concept, a hydrogen peroxide biosensor obtained by anchoring horseradish peroxidase (HRP) at a glassy carbon electrode (GCE) modified with the nanohybrid prepared by sonication of 2.0 mg mL- 1 MWCNTs and 0.50 mg mL- 1 SB-dBA in N,N-dimethyl formamide (DMF) for 30 min. The hydrogen peroxide biosensing was performed at -0.050 V in the presence of 5.0 x 10-4 M hydroquinone. The analytical characteristics of the resulting biosensor are the following: linear range between 0.175 mu M and 6.12 mu M, detection limit of 58 nM, and reproducibility of 2.0 % using the same nanohybrid (6 biosensors), and 9.0 % using three different nanohybrids. The sensor was successfully used to quantify hydrogen peroxide in enriched milk and human blood serum samples and in a commercial disinfector.
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    Functionalization using biocompatible carboxylated cyclodextrins of iron-based nanoMIL-100
    (2021) Carmona, Thais; Marco, Jose F.; Gimenez-Marques, Monica; Canon-Mancisidor, Walter; Gutierrez-Cutino, Marlen; Hermosilla-Ibanez, Patricio; Perez, Edwin G.; Minguez Espallargas, Guillermo; Venegas-Yazigi, Diego
    Here we report the first example of nanoMIL-100 particles modified with monomeric cyclodextrin derivatives of different length by exploiting strong interactions between non-saturated iron trimers at the external surface and carboxylate functionalities located at the end of biocompatible and flexible linkers of cyclodextrins. The main results revealed that, after the functionalization, the cyclodextrins are selectively located at the external surfaces covering the nanoparticles. Z potential measurements show that this functionalization induced changes respect to the bare nanoMIL-100 particles, however, the presence of the cyclodextrins does not modify the size neither porosity of the nanoparticles. The amount of cyclodextrins attached, investigated by thermogravimetry, increases with the length of the linker between CD cavity and nanoparticle surface, reaching up a 9 % wt. Auger spectroscopy suggested a clear predominant sp(3) character after the functionalizations (vs. sp(2) predominance in the unmodified nanoMIL-100). This study supposes the creation of an alternative family of hybrids based on carboxylated monomeric cyclodextrins.