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  1. Home
  2. Browse by Author

Browsing by Author "Guajardo-Leiva, Sergio"

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    Active Crossfire Between Cyanobacteria and Cyanophages in Phototrophic Mat Communities Within Hot Springs
    (2018) Guajardo-Leiva, Sergio; Pedros-Alio, Carlos; Salgado, Oscar; Pinto, Fabian; Diez, Beatriz
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    Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula
    (2019) Alarcon Schumacher, Tomas; Guajardo-Leiva, Sergio; Antón, Josefa; Diez Moreno, Beatriz
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    Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities
    (2022) Salgado Salgado, Oscar; Guajardo-Leiva, Sergio; Moya-Beltrán, Ana; Barbosa, Carla; Ridley, Christina; Tamayo-Leiva, Javier; Quatrini, Raquel; Mojica, Francisco; Díez, Beatriz
    The Cas1 protein is essential for the functioning of CRISPR-Cas adaptive systems. However, despite the high prevalence of CRISPR-Cas systems in thermophilic microorganisms, few studies have investigated the occurrence and diversity of Cas1 across hot spring microbial communities. Phylogenomic analysis of 2,150 Cas1 sequences recovered from 48 metagenomes representing hot springs (42–80°C, pH 6–9) from three continents, revealed similar ecological diversity of Cas1 and 16S rRNA associated with geographic location. Furthermore, phylogenetic analysis of the Cas1 sequences exposed a broad taxonomic distribution in thermophilic bacteria, with new clades of Cas1 homologs branching at the root of the tree or at the root of known clades harboring reference Cas1 types. Additionally, a new family of casposases was identified from hot springs, which further completes the evolutionary landscape of the Cas1 superfamily. This ecological study contributes new Cas1 sequences from known and novel locations worldwide, mainly focusing on under-sampled hot spring microbial mat taxa. Results herein show that circumneutral hot springs are environments harboring high diversity and novelty related to adaptive immunity systems.
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    Seasonal and Spatially Distributed Viral Metagenomes from Comau Fjord (42°S), Patagonia
    (2023) Castro-Nallar, Eduardo; Berrios-Farias, Valentin; Diez, Beatriz; Guajardo-Leiva, Sergio
    Viruses are key players in marine environments, affecting food webs and biogeochemical cycles. We present 48 viral metagenomes and 5,656 viral operational taxonomic units (vOTUs) from Comau Fjord, Patagonia (42 degrees S), to understand viral-mediated processes in coastal and estuarine waters. These data represent a spatial (35-km transect, two depths) and seasonal (winter and fall) data set.

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