Browsing by Author "Guajardo, Eduardo"

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    Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
    (2013) Contreras-Porcia, Loretto; Lopez-Cristoffanini, Camilo; Lovazzano, Carlos; Rosa Flores-Molina, Maria; Thomas, Daniela; Nunez, Alejandra; Fierro, Camila; Guajardo, Eduardo; Correa, Juan A.; Kube, Michael; Reinhardt, Richard
    In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/ proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.
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    Spatio-temporal variation in the composition of the macroalgae assemblage of the intertidal rocky zone from maitencillo, valparaíso, central coast of Chile
    (2018) Meynard, Andrés; Ramírez, María Eliana; González Campero, María Constanza; Zapata, Javier; Castañeda, Francisco; Salas, Nicolás; Espinoza, Camila; Guajardo, Eduardo; Bulboa Contador, Cristian; Contreras Porcía, Loretto Matilde; Betancourtt, Claudia; Anguita, Cristóbal; Latorre, Nicolás; Núñez, Alejandra; Fierro, Camila