Browsing by Author "Grau, Arturo E."

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    Clinical Presentation and Causes of Recurrent Corneal Erosion Syndrome: Review of 100 Patients
    (2014) Diez-Feijoo, Elio; Grau, Arturo E.; Abusleme, Eugenia I.; Duran, Juan A.
    Purpose: To study the clinical features and etiology of recurrent corneal erosion syndrome (RCES).
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    Evidence of autophagic vesicles in a patient with Lisch corneal dystrophy
    (2020) Grau, Arturo E.; Gonzalez, Sergio; Zoroquiain, Pablo; Gonzalez, Pablo A.; Khaliliyeh, Daniela; Morselli, Eugenia; Cortes, Dennis
    Lisch corneal dystrophy is a rare corneal disease characterized by the distinctive feature of highly vacuolated cells. Although this feature is important, the nature of these vacuoles within corneal cells remains unknown. Here, we sought to analyze corneal cells from a patient diagnosed with Lisch dystrophy to characterize the vacuoles within these cells. Analyses using histopathology examination, confocal microscopy, and transmission electron microscopy were all consistent with previous descriptions of Lisch cells. Importantly, the vacuoles within these cells appeared to be autophagosomes and autolysosomes, and could be stained with an anti-microtubule-associated protein 1A/1B-light chain 3 (LC3) antibody. Taken together, these findings indicate that the vacuoles we observed within superficial corneal cells of a patient with Lisch corneal dystrophy constituted autophagosomes and autolysosomes; this finding has not been previously reported and suggests a need for further analyses to define the role of autophagy in this ocular disease.
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    Low Expression of Vitamin D Receptor in Patients With Dry Eye Disease
    (2024) Vieira, Antonia; Meza, Javiera; Garreton, Rodolfo; Grau, Arturo E.; Zoroquiain, Pablo
    Purpose:This study aimed to compare the expression of vitamin D receptor (VDR) on the ocular surface of patients with dry eye disease (DED) and controls without ophthalmological pathologies.Methods:Patients with DED without previous treatment were studied and compared with healthy subjects. Ocular Surface Disease Index assessement and ocular surface cytology were performed in all patients. The immunohistochemical expression of VDR was evaluated using fully automated immunohistochemistry. The evaluation involved multiplying the percentage of nuclear-labeled cells (0-100) by their intensity (0-3), resulting in a score ranging from 0 to 300 (VDR H-score). Squamous metaplasia was morphologically evaluated using liquid-phase cytology with Papanicolaou/periodic acid-Schiff staining using Nelson's grade (scale 0-3, higher grade to higher metaplastic change).Results:Eighteen patients with DED without previous treatment and 10 healthy subjects were studied. Squamous metaplasia was observed in 74% of patients with DED, in contrast to 0% in the control group. In patients with DED, there was a lower expression of VDR than in the control group (VDR H-score: 11.2 +/- 13.9 vs. 80.9 +/- 56; P = 0.0001). Furthermore, an inverse correlation was observed between Nelson grade and VDR H-score (P = 0.0001, rs = -0.71). No correlation was observed between Ocular Surface Disease Index and VDR H-score.Conclusions:This is the first study to evaluate the VDR in patients with DED. These patients presented with a lower expression of VDR than healthy subjects. No correlation was found with more severe symptoms. Patients with DED also had a higher frequency of squamous metaplasia.