Browsing by Author "Gonzalez, Gustavo"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Peroxynitrite oxidizes erythrocyte membrane band 3 protein and diminishes its anion transport capacity
    (2007) Celedon, Gloria; Gonzalez, Gustavo; Pino, Jose; Lissi, Eduardo A.
    We describe an altered membrane band 3 protein-mediated anion transport in erythrocytes exposed to peroxynitrite, and relate the loss of anion transport to cell damage and to band 3 oxidative modifications. We found that peroxynitrite down-regulate anion transport in a dose dependent relation (100-300 mu moles/1). Hemoglobin oxidation was found at all peroxynitrite concentrations studied. A dose-dependent band 3 protein crosslinking and tyrosine nitration were also observed. Band 3 protein modifications were concomitant with a decrease in transport activity. (-)-Epicatechin avoids band 3 protein nitration but barely affects its transport capacity, suggesting that both processes are unrelated. N-acetyl cysteine partially reverted the loss of band 3 transport capacity. It is concluded that peroxynitrite promotes a decrease in anion transport that is partially due to the reversible oxidation of band 3 cysteine residues. Additionally, band 3 tyrosine nitration seems not to be relevant for the loss of its anion transport capacity.