Browsing by Author "González, S."

Now showing 1 - 5 of 5
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    Biliary aminopeptidase-N and the cholesterol crystallization defect in cholelithiasis
    (British Medical Journal Publishing Group, 1995) Núñez, L.; Amigo Boker, Ludwig Peter; Mingrone, G.; Rigotti Rivera, Attilio Gianpietro; Puglielli, L.; Raddatz Echavarría, Alejandro; Pimentel González, Eduardo Fernando; Greco, A.; González, S.; Garrido Negri, Jorge; Miquel Poblete, Juan Francisco; Nervi Oddone, Flavio; Pontificia Universidad Católica de Chile. Departamento de Gastroenterología y Centro para la Prevención y tratamiento del Cáncer Digestivo; Institute of Clinical Medicine, Universita Cattolica del Sacro Cuore, Rome, Italy
    Several biliary proteins have cholesterol crystallisation promoting activity. One of these glycoproteins is aminopeptidase-N, a canalicular ectoenzyme. This study attempted to localise aminopeptidase-N along the biliary tree, to assess its concentration in a series of 98 patients subjected to abdominal surgery, 40 of them without gap stones, and to correlate its concentration with cholesterol crystal formation time of gall bladder bile. Aminopeptidase-N was isolated from purified native biliary vesicles. A specific polyclonal rabbit anti-aminopeptidase-N antibody was prepared for quantitative immunoblotting and for immunolocalisation. Tissue was obtained from liver biopsy specimens and from gall bladders removed at surgery because of gall stone disease. Aminopeptidase-N was immunolocalised to the apical membranes of hepatocytes and to the apical pole of ductular and gall bladder mucosal cells. The nucleation time of gall bladder bile was mean (SD) 4 (3) days in the gall stone group, compared with 21 (18) days in the control group (p < 0.001). Total absolute biliary protein and aminopeptidase-N concentrations were similar in both the control and gall stone patients. There was a reciprocal significant correlation, however, between the nucleation time and the relative aminopeptidase-N concentration (r = -0.35, p < 0.01) only in the gall stone group of patients, This study shows that this apical transmembrane ectoenzyme with cholesterol crystallisation promoting activity is present along the biliary tree and the hepatocyte. These findings support the concept that high concentrations or qualitative changes of biliary aminopeptidase-N contribute to cholesterol gall stone formation.
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    Consenso de terminología en microscopia confocal de reflectancia en español mediante método Delphi
    (2023) Abarzúa, Alvaro; Banuls, J.; Cabo, H.; Carrera, C.; Gamo, R.; González, S.; Jaimes, N.; Navarrete Dechent, Cristián Patricio; Perez Anker J.; Roldan Marín, R.; Segura, S.; Yelamos, O.; Puig, S.; Malvehy, J.
    La terminología usada para describir los diferentes hallazgos en la microscopía confocal de reflectancia (MCR), tanto en lesiones melanocíticas, como en no melanocíticas se ha consensuado en inglés. En el presente trabajo, se proponen los términos en español que mejor interpretan estos conceptos ya descritos para la MCR, mediante el consenso de expertos de distintas nacionalidades de habla hispana y utilizando el método DELPHI para el acuerdo final. Se obtuvieron 52 términos en total, de los cuales 28 fueron para lesiones melanocíticas y 24 para lesiones no melanocíticas. El uso de la nomenclatura propuesta permitirá una homogeneización y mejor entendimiento de las estructuras; una descripción más estandarizada en los registros clínicos y una mejor interpretación de estos informes por otros dermatólogos.
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    End of life, death and dying in neonatal intensive care units in Latin America
    (2012) Fajardo, C. A.; González, S.; Zambosco, G.; Cancela, M. J.; Forero, L. V.; Venegas, M.; Baquero, H.; Lemus Varela, L.; Kattan Said, Alberto Javier; Wormald Langdon, Francisca; Solà, A.; Lantos, J.
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    Pediatric lung resection. A case series and evaluation of postoperative lung function
    (EDICIONES DOYMA, S.L., 2001) Caussade Larraín, Marie Solange; Zúñiga Rojo, Sergio Andrés; García, C.; González, S.; Campos, E.; Soto, G.; Zúñiga, F.; Sánchez Díaz, Ignacio
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    The potential utility of integrated reflectance confocal microscopy-optical coherence tomography for guiding triage and therapy of basal cell carcinomas
    (2020) Iftimia, N.; Sahu, A.; Córdova, M.; Maguluri, G.; Gill, M.; Alessi-Fox, C.; González, S.; Navarrete Dechent, Cristián Patricio; Marghoob, A.; Chen, C. S. J.; Rajadhyaksha, M.