Browsing by Author "González, Leticia"

Now showing 1 - 4 of 4
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    Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease
    (2025) Rivera, Katherine; González, Leticia; Parra, Laura; Oyarzún, Juan E.; Concepción-Alvarez, Alina; Costa de Camargo, Adriano; Bridi, Raquel; Rigotti, Attilio; Andia, Marcelo E.
    Red wine grape pomace (RWGP), a winemaking by-product rich in phenolics, flavonoids, and dietary fiber, has shown promise in mitigating cardiovascular disease (CVD), however, its mechanisms of action remain incompletely understood. This study comprehensively profiled the phenolic composition of RWGP—including free, esterified, etherified, and insoluble-bound fractions—and evaluated the effects of RWGP dietary supplementation on gut barrier integrity, inflammation, oxidative stress, and survival in SR-B1−/−ApoE-R61h/h mice, a model of diet-induced lethal ischemic heart disease. RWGP supplementation significantly improved survival rates and restored gut barrier function, as evidenced by lower plasma FITC-dextran and LPS levels, increased circulating ZO-1 levels, and reduced histopathological colon damage. In addition, RWGP reduced pro-inflammatory cytokines (IL-1𝛽) and showed a trend toward attenuating systemic oxidative stress (TBARS). Analysis of phenolic compounds indicated a significant presence of insoluble-bound phenolics. Nevertheless, the beneficial effects observed are likely attributable to the synergistic actions of RWGP’s complex phytochemical and fiber composition. These results highlight RWGP’s potential as a sustainable, gut-targeted functional food ingredient for CVD prevention and management.
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    Rho kinase activation in circulating leukocytes is related to hypertensive myocardial remodeling
    (2018) Ocaranza, María Paz; Fierro, Camila; Jalil Milad, Jorge; Moya, Jackeline; González, Leticia; Molina, Cristián; Mancilla, Cristián
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    Serum uric acid correlates with extracellular superoxide dismutase activity in patients with chronic heart failure
    (2008) Alcaíno, Hernán; Greig, Douglas; Chiong, Mario; Verdejo Pinochet, Hugo; Miranda, Rodrigo; Concepcion, Roberto; Vukasovic, José Luis; Díaz-Araya, Guillermo; Mellado Suazo, Rosemarie; García, Lorena; Salas, Daniela; González, Leticia; Godoy J., Iván; Castro Gálvez, Pablo Federico; Lavandero, Sergio
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    Sex-dependent differences on the impact of anti-inflammatory treatment in the progression of coronary artery disease in a murine model of lethal ischemic heart disease induced by diet
    (2023) Parra Núñez, Laura Macarena; Andía Kohnenkampf, Marcelo Edgardo; González, Leticia; Pontificia Universidad Católica de Chile. Facultad de Medicina
    Cardiovascular risk differs significantly between adult men and women. Therefore, it is expected that different treatments may affect both groups differently. We aim to compare sexdependent differences on survival and systemic inflammation in response to anti-inflammatory treatment using a diet-induced myocardial infarction mouse model. Method. Male and female SR-B1−/−ApoER61h/h mice, aged 2-3 months, were randomly assigned into two groups: Control (HFD-Control) and minocycline (HFD-MIN). Atherosclerosis was induced by feeding an atherogenic diet (15% fat, 1.25% cholesterol, 0.5% cholate). Minocycline was administered in the drinking water at a dose of 0.05 mg/mL. Female mice had a slightly better survival than male mice when fed an HFD (p=0.12). Minocycline improved survival in male by 35% (p=0.006) and by 33% in female p=0.01), without affecting total cholesterol levels. Male mice fed with HFD tended to have higher IL-6 levels than female mice (p=0.08). Minocycline significantly reduced IL-6 levels (p=0.04) and Ly6Chigh (p=0.006) and increased the Ly6Clow subset (p=0.006). Male and female fed with HFD clustered in different groups by analyzing inflammatory parameters by PCA; however, after minocycline intervention, were indistinguishable. High fat diet decreased survival and caused early death in this animal model, however, females had slightly better survival than male mice. Minocycline treatment improved survival in both groups although it did not affect their cholesterol levels. Males showed higher inflammatory serum biomarkers than females, and minocycline treatment showed a higher impact on systemic anti-inflammation in male mice than female, by reducing plasma IL-6 levels and shifting toward a more "reparative" phenotype on circulating monocyte subsets.