Browsing by Author "González, B."

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    Comparative analysis between nanorods and nanowires by using depolarized and diffuse light
    (2021) Valente, P.; Seré, A.; Pereyra, C. J.; Campo, L.; Spera, E.; Castillo Rodríguez, Judith; Hevia, Samuel; del Río, R.; Ramírez, D.; Riveros, G.; Álvarez, K.; González, B.; Marotti, R. E.; Dalchiele, E. A.
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    Culture dependent and independent analyses of bacterial communities involved in copper plumbing corrosion
    (2010) Pavissich Bartolozzi, Juan Pablo; Vargas Cucurella, Ignacio Tomás; González, B.; Pastén González, Pablo Arturo; Pizarro Puccio, Gonzalo E.
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    Design and evaluation of a Hg(II) sensor based on the response of a poly(3,4-ethylenedioxythiophene) modified electrode
    (2014) Valle de la Cortina, María Angélica del; Llanquileo Melgarejo, Paula Iris; Díaz, Fernando R.; Faúndez Cáceres, Mario; Hernández Díaz, Loreto Andrea; González, B.
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    Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile
    (2021) Díaz-Rullo, J.; Rodríguez-Valdecantos, G.; Torres-Rojas, F.; Cid, L.; Vargas Cucurella, Ignacio Tomas; González, B.; González-Pastor, J.E.; CEDEUS (Chile)
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    Molecular and population analyses of a recombination event in the catabolic plasmid pJP4
    (2006) Larraín-Linton, Juanita; De la Iglesia, R.; Melo Ledermann, Francisco Javier; González, B.
    Cupriavidus necator JMP134(pJP4) harbors a catabolic plasmid, pJP4, which confers the ability to grow on chloroaromatic compounds. Repeated growth on 3-chlorobenzoate (3-CB) results in selection of a recombinant strain, which degrades 3-CB better but no longer grows on 2,4-dichlorophenoxyacetate (2,4-D). We have previously proposed that this phenotype is due to a double homologous recombination event between inverted repeats of the multicopies of this plasmid within the cell. One recombinant form of this plasmid (pJP4-F3) explains this phenotype, since it harbors two copies of the chlorocatechol degradation tfd gene clusters, which are essential to grow on 3-CB, but has lost the tfdA gene, encoding the first step in degradation of 2,4-D. The other recombinant plasmid (pJP4-FM) should harbor two copies of the tfdA gene but no copies of the tfd gene clusters. A molecular analysis using a multiplex PCR approach to distinguish the wild-type plasmid pJP4 from its two recombinant forms, was carried out. Expected PCR products confirming this recombination model were found and sequenced. Few recombinant plasmid forms in cultures grown in several carbon sources were detected. Kinetic studies indicated that cells containing the recombinant plasmid pJP4-FM were not selectable by sole carbon source growth pressure, whereas those cells harboring recombinant plasmid pJP4-F3 were selected upon growth on 3-CB. After 12 days of repeated growth on 3-CB, the complete plasmid population in C. necator JMP134 apparently corresponds to this form. However, wild-type plasmid forms could be recovered after growing this culture on 2,4-D, indicating that different plasmid forms can be found in C. necator JMP134 at the population level.
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    Novel α-ketoglutarate dioxygenase tfdA-related genes are found in soil DNA after exposure to phenoxyalkanoic herbicides
    (2010) Gazitúa, M. C.; Slater, A. W.; Melo Ledermann, Francisco Javier; González, B.
    Phenoxyalkanoic herbicides such as 2,4-dichlorophenoxyacetate (2,4-D), 2,4-dichlorophenoxybutyrate (2,4-DB) or mecoprop are widely used to control broad-leaf weeds. Several bacteria have been reported to degrade these herbicides using the α-ketoglutarate-dependent, 2,4-dichlorophenoxyacetate dioxygenase encoded by the tfdA gene, as the enzyme catalysing the first step in the catabolic pathway. The effects of exposure to different phenoxyalkanoic herbicides in the soil bacterial community and in the tfdA genes diversity were assessed using an agricultural soil exposed to these anthropogenic compounds. Total community bacterial DNA was analysed by terminal restriction fragment length polymorphism of the 16S rRNA and the tfdA gene markers, and detection and cloning of tfdA gene related sequences, using PCR primer pairs. After up to 4 months of herbicide exposure, significant changes in the bacterial community structure were detected in soil microcosms treated with mecoprop, 2,4-DB and a mixture of both plus 2,4-D. An impressive variety of novel tfdA gene related sequences were found in these soil microcosms, which cluster in new tfdA gene related sequence groups, unequally abundant depending on the specific herbicide used in soil treatment. Structural analysis of the putative protein products showed small but significant amino acid differences. These tfdA gene sequence variants are, probably, required for degradation of natural substrate(s) structurally related to these herbicides and their presence explains self-remediation of soils exposed to phenoxyalkanoic herbicides.
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    Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN
    (2013) Zúñiga, A.; Poupin Swinburn, María Josefina; Donoso, R.; Ledger, T.; Guiliani, N.; Gutiérrez Ilabaca, Rodrigo Antonio; González, B.
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    Synthesis and electrochemical characterization of new ruthenium-terthiophene complexes
    (2015) González, B.; Valle Delaware la Cortina, María Angélica del; Díaz, F. R.; Espinosa Bustos, Christian Marcelo; Ramírez, A.; Hernández, L. A.; González, B.; Valle Delaware la Cortina, María Angélica del; Díaz, F. R.; Espinosa Bustos, Christian Marcelo; Ramírez, A.; Hernández, L. A.
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    Taxonomic and Biogeographical Status of Guanaco Lama Guanicoe (Artiodactyla, Camelidae)
    (2006) González, B.; Palma Vásquez, Ramón Eduardo
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    Using fMRI connectivity to define a treatment-resistant form of post-traumatic stress disorder
    (2019) Etkin, A.; Maron-Katz, A.; Wu, W.; Fonzo, G. A.; Huemer, J.; Vertes, P. E.; Patenaude, B.; Richiardi, J.; Goodkind, M. S.; Crossley, Nicolás; Keller, C. J.; Ramos-Cejudo, J.; Zaiko, Y. V.; Peng, K. K.; Shpigel, E.; Longwell, P.; Toll, R. T.; Thompson, A.; Zack, S.; González, B.; Edelstein, R.; Chen, J. Y.; Akingbade, I.; Weiss, E.; Hart, R.; Mann, S.; Durkin, K.; Baete, S. H.; Boada, F. E.; Genfi, A.; Autea, J.; Newman, J.; Oathes, D. J.; Lindley, S. E.; Abu-Amara, D.; Arnow, B. A.; Hallmayer, J.; Fossati, S.; Rothbaum, B. O.; Marmar, C. R.; Bullmore, E. T.; O'Hara, R.