Browsing by Author "Godoy, Paula"

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    Oral polio vaccine in infants does not interfere in detection of enterovirus in blood
    (SOC CHILENA INFECTOLOGIA, 2013) Gonzalez, Marcela; Sandoval, Carmen; Valenzuela, Patricia; Montecinos, Luisa; Martinez, Constanza; Godoy, Paula; Abarca, Katia
    Introduction: There is not known if a viraemia post-oral polio vaccine (OPV) is detectable by modern molecular techniques. Such viraemia could affect the performance of the real time-polymerase chain reaction (PCR) for non polio enterovirus (EV) detection, technique of growing clinical use for the study of febrile infants. Objective: To determine viraemia post-first dose of OPV in healthy infants, by molecular techniques. Patients and Methods: 50 infants less than three months without previous VPO were randomized in 5 groups: a control group with prevaccination blood sample (BS), group 1 BS at day 2, group 2 BS at day 4, group 3, BS at day 6 and group 4, BS at day 8 post-vaccination. Conventional and specific PCR for poliovirus and real time PCR for non polio EV were performed in BS and in OPV samples. Results: No genetic material of poliovirus was detected in any infant, while in 9 of them (18%) non polio EV was identified. Real time PCR for EV did not amplify poliovirus from OPV samples. Discussion: Results suggest that no post VPO viraemia detectable by molecular methods exists. Considering that real time PCR for EV does not allow to identify polio virus, no false positives of the test are expected as a result of a recent VPO vaccination. We documented presence of non polio EV in blood of healthy asymptomatic infants.
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    Range expansion of Oligoryzomys longicaudatus (Rodentia, Sigmodontinae) in Patagonian Chile, and first record of Hantavirus in the region
    (2009) Belmar-Lucero, Sebastian; Godoy, Paula; Ferres, Marcela; Vial, Pablo; Eduardo Palma, R.
    At present, 20 species of Oligoryzomys (Rodentia, Sigmodontinae) are recognized in the Neotropical region, most of them distinguished by their karyotypes, which fluctuates between 46-70 chromosomes. Two species are currently recognized in Chile, Oligoryzomys longicaudatus (Bennet, 1832: "colilargo" or the long-tailed pygmy rice rat: 2n = 56), which ranges from 27 degrees to approximately 51 degrees S, and O. magellanicus (Bennet, 1 836 Magellanic pygmy rice rate 2n = 54), south of 51 degrees S in the Patagonian region of Chile and Argentina. As part of an ongoing research on the southern Patagonia of Chile. we report the results Of small mammal samplings in six localities. We karyotyped 28 specimens and we also sequenced the hypervariable mtDNA region I in 22 individuals. aligning these sequences With an Under development phylogeny of O. longicaudatus. We also evaluated the serology and viral charge in all captured specimens to detect the presence of antibodies to Andes virus (ANDV) through Strip Immunoblot Assay (SIA), and of viral genome by RT-PCR. The results consistently showed that the karyotype of southern Patagonia specimens was 2n = 56, equal to that of O. longicaudatusand that individuals from this area (to not differentiate phylogenetically from those of the northern range of distribution. In addition, the serology showed the presence of antibodies IgG anti-ANDV and of viral genome in heart, kidney, spleen, and lungs S of a single specimen of Oligoryzomys from the locality of Fuerte Bulnes in the Magallanes region. We conclude that all specimens trapped south of 51 degrees S correspond to Oligoryzomys longicaudatus thus expanding the distribution of this speciei from 51 degrees to at least 55 degrees S. The results also extended the distribution of the Andes strain of Hantavirus to Southernmost Patagonia.