Browsing by Author "Giovagnoli-Vicuna, Claudia"

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    Annona cherimola Seed Extracts Trigger an Early Apoptosis Response and Selective Anticlonogenic Activity against the Human Gastric Carcinoma Cell Line SNU-1
    (2023) Macuer-Guzman, Johan; Giovagnoli-Vicuna, Claudia; Bernal, Giuliano; Lobos-Gonzalez, Lorena; de la Fuente-ortega, Erwin; Araya-Castillo, Michael; Ibanez, Cristian
    The aim of this study was to evaluate, for the first time, the antiproliferative, apoptotic and diminishing effects of the anchored growth-independent capacity of an ethanol macerate extract from the Annona cherimola seed (EMCHS) in the human gastric cancer cell line SNU-1. The cells treated with EMCHS (20 mu g/mL) significantly reduced the capacity to form clones of the tumor cell. Moreover, 50 mu g/mL of EMCHS extract induced apoptosis, as was shown by the Annexin-V assay. UHPLC-MS/MS analysis detected two acetogenins (Annonacinone and Annonacin) in the EMCHS, which could be largely responsible for its selective antiproliferative effect. The identification of fatty acids by GC-FID showed the presence of eight fatty acids, among which was, oleic acid, which has recognized activity as an adjuvant in antitumor treatments. Taken together, our results indicate that the EMCHS seems promising for use as a natural therapy against gastric cancer disease.
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    Effect of Extraction Methods and In Vitro Bio-Accessibility of Microencapsulated Lemon Extract
    (2022) Giovagnoli-Vicuna, Claudia; Briones-Labarca, Vilbett; Soledad Romero, Maria; Giordano, Ady; Pizarro, Sebastian
    The extraction of bioactive compounds from fruits, such as lemon, has gained relevance because these compounds have beneficial properties for health, such as antioxidant and anticancer properties; however, the extraction method can significantly affect these properties. High hydrostatic pressure and ultrasound, as emerging extraction methods, constitute an alternative to conventional extraction, improving extractability and obtaining extracts rich in bioactive compounds. Therefore, lemon extracts (LEs) were obtained by conventional (orbital shaking), ultrasound-assisted, and high-hydrostatic-pressure extraction. Extracts were then microencapsulated with maltodextrin at 10% (M10), 20% (M20), and 30% (M30). The impact of microencapsulation on LEs physicochemical properties, phenolics (TPC), flavonoids (TFC) and relative bio-accessibility (RB) was evaluated. M30 promoted a higher microencapsulation efficiency for TPC and TFC, and a longer time required for microcapsules to dissolve in water, as moisture content, water activity and hygroscopicity decreased. The RBs of TPC and TFC were higher in microcapsules with M30, and lower when conventional extraction was used. The data suggest that microencapsulated LE is promising as it protects the bioactivity of phenolic compounds. In addition, this freeze-dried product can be utilized as a functional ingredient for food or supplement formulations.
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    Effects of optimized osmotic vacuum impregnation on quality properties of red abalone (Haliotis rufescens) drying
    (2023) Pizarro-Oteiza, Sebastian; Giovagnoli-Vicuna, Claudia; Briones-Labbarca, Vilbett; Salazar, Fernando
    The aim of this work was to analyze the quality of red abalone dehydrated under osmotic vacuum impregnation (OVI) as a pretreatment to the convection drying process. Response surface methodology was used to optimize osmotic impregnation conditions. Then, diffusional parameters of salt (D-se) and water (D-we), the drying kinetics, and quality characteristics were analyzed. According to the RSM, the optimized impregnation conditions were 92 mbar at 20% NaCl for 14 min. The D-se and D-we diffusion parameters for OVI were 7.72 and 4.14 x 10(-10) (m(2)/s), respectively. The drying kinetics of the OVI sample obtained a higher D-we value than conventional impregnation. The quality showed a more tenderness product, higher rehydration and water holding capacity and less non-enzymatic browning and color change. Therefore, OVI is a promising alternative to conventional impregnation, the method currently used for pretreatment seafood which requires a long processing time.
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    Encapsulation of papaya seed oil in casein-alginate-based shell materials
    (2024) Canas-Sarazia, Rail; Briones-Labarca, Vilbett; Giovagnoli-Vicuna, Claudia
    This study aimed to develop papaya seed oil macrocapsules using ionotropic gelation encapsulation with casein and sodium alginate as shell materials. Mechanical stability and physical properties, assessed via response surface methodology, were used to determine optimal conditions. Bioaccessibility and oil release post in -vitro gastrointestinal digestion were also quantified. Independent variables (papaya seed oil concentration, calcium chloride percentage, and pH) were examined, revealing correlation coefficients (R2) of 0.938, 0.957, 0.954, and 0.845 for elastic limit, Young modulus, oil loss capacity, and capsule diameter, respectively. Optimal encapsulation conditions were identified as 60 % papaya seed oil, 0.5 % calcium chloride (w/v), and pH 2, resulting in macrocapsules with an elastic limit of 176.84 Pa, Young modulus of 4.44 Pa, oil loss capacity of 19.81 %, and a capsule diameter of 2.727 mm. In -vitro studies indicated a target oil release of 52.23 %, primarily in the intestinal phase, with a bioaccessibility of 75.36 %. This study underscores the impact of encapsulation process conditions on macrocapsule properties and highlights the potential application of casein alginate macrocapsules as oral delivery vehicles for lipophilic compounds in the food and nutraceuticals industry.
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    Microencapsulation of Chilean Papaya Waste Extract and Its Impact on Physicochemical and Bioactive Properties
    (2023) Fuentes, Yihajara; Giovagnoli-Vicuna, Claudia; Faundez, Mario; Giordano, Ady
    The microencapsulation of bioactive extracts of Chilean papaya waste, including both seeds and skin, was investigated. Papaya waste extract microcapsules utilizing maltodextrin at 10% (MD10), 20% (MD20), and 30% (MD30) (w/v) as the wall material through the freeze-drying process were obtained, and subsequently their physicochemical, antioxidant, and antimicrobial properties were evaluated. The TPC efficiency and yield values achieved were more than 60% for the microencapsulated seed and skin extracts, respectively. The best results for phenolic and antioxidant compounds were found in the microencapsulated seed extract with MD20, with a value of 44.20 +/- 3.32 EAG/g DW for total phenols and an antioxidant capacity of 12.0 +/- 0.32 mol ET/g DW for the DPPH and 236.3 +/- 4.1 mol ET/g DW for the FRAP assay. In addition, the seed and skin samples reduced ROS generation in H2O2-treated Hek293 cells. In terms of antimicrobial activity, values ranging from 7 to 15 mm of inhibitory halos were found, with the maximum value corresponding to the inhibition of S. aureus, for both microencapsulated extracts. Therefore, the successful microencapsulation of the waste bioactive extracts (seed and skin) with the demonstrated antimicrobial and antioxidant properties highlight the bioactivity from Chilean papaya waste resources.