Browsing by Author "Giordano Villatoro, Ady"

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    Bioactive compounds and antibacterial properties of monofloral Ulmo honey
    (2020) Velásquez, P; Montenegro Rizzardini, Gloria; Leyton, Felipe; Ascar, Loreto; Ramírez, O; Giordano Villatoro, Ady
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    Bioactive polyphenols and antioxidant capacity of Azara petiolaris and Azara integrifolia Honeys
    (2018) Giordano Villatoro, Ady; Retamal, Mauricio; Leyton, Felipe; Martinez, Patricia; Bridi, Raquel; Velasquez, Patricia; Montenegro Rizzardini, Gloria
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    Bioactivities of phenolic blend extracts from Chilean honey and bee pollen
    (2019) Velásquez, Patricia; Montenegro Rizzardini, Gloria; Giordano Villatoro, Ady; Retamal, M.; Valenzuela, L. M.
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    Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds : an undervalued Chilean resource
    (2020) Cabrera Barjas, G.; Quezada, A.; Bernardo, Y.; Moncada, M.; Zuniga, E.; Wilkens, M.; Giordano Villatoro, Ady; Nesic, A.; Delgado, N.
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    Determination of sunscreen compounds in water samples by silicone rod extraction
    (2013) Giordano Villatoro, Ady; Richter, Pablo; Leiva, K.; Ascar, Loreto
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    Differences between phenolic content and antioxidant capacity of quillay Chilean honeys and their separated phenolic extracts
    (2017) Bridi, Raquel; Nuñez-Quijada, Gabriel; Aguilar, Patricia; Martínez, Patricia; Lissi, Eduardo; Giordano Villatoro, Ady; Montenegro Rizzardini, Gloria
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    Estrategias de screening basadas en espectroscopía Raman para la caracterización de mieles nativas chilenas: estudio de azúcares adulterantes y pesticidas neonicotinoides
    (2023) Leyton Soto, Felipe Arturo; Isaacs Casanova, Mauricio; Giordano Villatoro, Ady; Pontificia Universidad Católica de Chile. Escuela de Química
    Actualmente, la búsqueda de metodologías analíticas rápidas, sencillas y económicas para la determinación de adulteración y presencia de contaminantes en matrices ambientales es fundamental. La espectroscopía Raman surge como una alternativa, ya que proporciona una huella dactilar vibracional única que permite identificar una molécula y además mediante el uso de plataformas SERS mejora la intensidad de señales características de grupos de alta densidad electrónica. En este trabajo, para determinar el contenido de azúcares adulterante en muestras de miel, se adulteraron intencionalmente muestras de miel auténtica en proporciones desde 10 a 90% con soluciones de glucosa, fructosa, sacarosa y un jarabe comercial. De esta forma, a partir de la espectroscopía Raman junto con algoritmos quimiométricos multivariados de tipo PCA, SIMCA y PLSR se determinó la autenticidad de un grupo de muestras de mieles Chilenas, además de poder clasificar y cuantificar a nivel de screening el contenido de adulterante en muestras de miel. Por otro lado, para la determinación de pesticidas se sintetizaron sustratos SERS con nanohojas de plata (AgNS) sobre una superficie de cobre, lo que produjo un fuerte efecto SERS y una intensidad de señal reproducible a partir de la molécula de sonda de azul de metileno en un tiempo de reacción óptimo de 1 min. Se realizó un análisis cuantitativo del plaguicida imidacloprid en solución variando la concentración del plaguicida, obteniendo espectros con señales características y detectando este analito a 0,02 µmol L 1, para luego realizar una extracción de QuEChERS en miel obteniendo un porcentaje de recuperación del 75%, permitiendo establecer una metodología de screening. Además, se llevaron a cabo simulaciones de la teoría funcional de la densidad para de esta forma poder realizar una asignación de los modos vibracionales del imidacloprid.
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    Flavonoids and triterpenes isolated from Eucryphia cordifolia (Cunoniaceae)
    (2022) Viteri Espinoza, Rafael Antonio; Giordano Villatoro, Ady; Montenegro Rizzardini, Gloria; Zacconi, Flavia C. M.
    The phytochemical study of the leaves of Eucryphia cordifolia (Cunoniaceae) allowed for the isolation of six compounds: the triterpenes betulin (1) and 28-acetoxy betulin (3), the steroid β-sitosterol (2), the flavonol quercetrin (4), the flavanonol astilbine (5) and the flavanol catechin (6). The structures of the isolated compounds were clearly assigned through 1D and 2D-NMR, MS studies, and compared to previously described data. This is the first report on the isolation of compounds 1–4 and 6 from the genus Eucryphia. The chemotaxonomic importance of the isolated compounds within the family of Cunoniaceae-related plants is discussed.
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    Ibuprofen, Carbamazepine and β-Estradiol Determination Using Thin-Film Microextraction and Gas Chromatography-Mass Spectrometry
    (2016) Giordano Villatoro, Ady; Vásquez, José; Retamal, Mauricio; Ascar, Loreto
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    International Regulations of Propolis Quality : Required Assays do not Necessarily Reflect their Polyphenolic‐Related In Vitro Activities
    (2015) Bridi, Raquel; Montenegro Rizzardini, Gloria; Nunez-Quijada, G.; Giordano Villatoro, Ady; Moran-Romero, M.F.; Jara-Pezoa, I.; Speisky, Hernán; Atala, E.; López Alarcón, Camilo Ignacio
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    LC-MS/MS analysis of neonicotinoid insecticides : residue findings in chilean honeys
    (2018) Bridi, Raquel; Larena, Arturo; Nunez Pizarro, Paula; Giordano Villatoro, Ady; Montenegro Rizzardini, Gloria
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    Metabolomic Profiling and Antioxidant Properties of Chilean Eucryphia cordifolia Cav.: Insights from Leaves, Flowers, and Monofloral Honey
    (2025) Viteri Espinoza, Rafael Antonio; Giordano Villatoro, Ady; Montenegro Rizzardini, Gloria; Simirgiotis, Mario J.; Zacconi, Flavia C. M.
    This study aimed to characterize the metabolomic profile of monofloral honey from Eucryphia cordifolia (ulmo) and evaluate the potential transfer of bioactive compounds from the plant parts, including the leaves and flowers, to the honey. Using UHPLC/ Q-TOF-MS analysis, various flavonoids and phenolic acids were identified and quantified in extracts from the leaves, flowers, and honey from E. cordifolia. Given their rich polypheAcademicEditor: Elisabeta Irina Geana Received: 11January2025 Revised: 24February2025 Accepted: 26February2025 Published: 28 February2025 Citation: Viteri, R.; Giordano, A.; Montenegro,G.;Simirgiotis, M.J.; Zacconi, F.C. MetabolomicProfiling andAntioxidantProperties ofChilean Eucryphia cordifolia Cav.: Insights from Leaves, Flowers, andMonofloral Honey. Antioxidants 2025, 14, 292. https://doi.org/10.3390/ antiox14030292 Copyright: ©2025bytheauthors. Licensee MDPI,Basel,Switzerland. This article is an open access article distributed under the termsand conditions of the Creative Commons Attribution (CC BY)license (https://creativecommons.org/ licenses/by/4.0/). nolic composition, E. cordifolia leaves were included in this study to assess their potential contribution to the antioxidant properties and chemical markers of ulmo honey. Additionally, the polyphenolic compounds in honey samples were quantified. Chromatographic analysis via UHPLC-MS/MS revealed that ulmo honey contains phenolic acids such as gallic, syringic, ferulic, chlorogenic, caffeic, and coumaric acid, as well as flavonoids including pinocembrin, quercetin, luteolin, kaempferol, epicatechin, apigenin, and isorhamnetin. The results indicate that pinocembrin and gallic acid are the main chemical markers of ulmo honey, while isorhamnetin could complement its characterization as a complementary marker. UHPLC/Q-TOF-MS analysis was also utilized to compare the compounds present in the honey with those found in the plant parts (leaves and flowers), respectively. A total of 10 shared compounds were identified, 9 of which were preliminarily identified, while 1remainsunknown. Notably,dihydroquercetin3-O-rhamnoside,quercetin3-O-rhamnoside, cyanidin 3-(p-coumaroyl)-glucoside, and eupatorin were detected in ulmo honey for the f irst time. Along with gallic acid, pinocembrin, and isorhamnetin, these compounds could contribute to a characteristic fingerprint for identifying the botanical origin of the honey. Overall, these findings provide valuable insights into the chemical composition of ulmo honey and its potential application as a functional product with antioxidant properties.
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    Mobility of nonsteroidal Anti-inflammatory drugs in soils with and without amendment of biosolid
    (2017) Ascar. L.; Ahumada, I.; Morales, N.; Garrido, T.; Giordano Villatoro, Ady; Leiva, K.
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    Nutritional Composition and Polyphenolic Content of Edible Peumus boldus Mol. Fruits.
    (2017) Velasquez, P.; Sandoval, I.; Giordano Villatoro, Ady; Gomez Unjidos, Miguel Crescencio; Montenegro Rizzardini, Gloria
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    Pharmaceutical compounds determination in water samples: Comparison between solid phase extraction and stir bar sorptive extraction
    (2017) Suazo, Fernanda; Vásquez, José; Retamal, Mauricio; Ascar, Loreto; Giordano Villatoro, Ady
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    Pharmacokinetics of methadone in adult patients undergoing cardiac surgery with cardiopulmonary bypass
    (2024) Salas, Wilbaldo E.; Cortinez, Luis Ignacio; López Barreda, Rodrigo; Rolle, Augusto; Elgueta, Francisca; Godoy, César; Giordano Villatoro, Ady; Contreras Ibacache, Víctor; Anderson, Brian J.
    Cardiopulmonary bypass (CPB) induces profound physiological changes that may alter the pharmacokinetics of methadone. We aimed to describe the pharmacokinetics of an intravenous bolus of methadone racemate in adult patients undergoing heart surgery with CPB. METHODS: We prospectively studied 29 patients aged 45 to 75 years scheduled for cardiac surgery with CPB who received methadone 0.2 mg/kg after anesthesia induction. Arterial blood samples (n = 10) were taken, before, during, and after CPB. Pharmacokinetic analysis was undertaken using nonlinear mixed effects models. RESULTS: All patients completed the study. The median [interquartile range] methadone concentrations decreased from 34.8 [23.9–48.2] ng/mL (10 minutes before CPB) to 18.2 [9.9–26] ng/mL after 60 minutes of CPB (P < .001). A 3-compartment model adequately described the observed changes in methadone concentrations. The influence of CPB on methadone pharmacokinetics was best described by hemodilution in a fixed volume of 1.5 L (CPB circuit volume) and by sequestration from the CPB components (CLSEQ = 93.4 L/h, 95%CI 59–124, P < .01). The observed effect of CPB in methadone pharmacokinetics can be compensated by giving a supplementary bolus dose of 0.05 mg/kg at the end of CPB. CONCLUSIONS: Our results confirmed a decay in methadone concentrations during CPB, which, in our modeling analysis, was attributed to hemodilution and sequestration within the CPB components.
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    Polyphenolic distribution in organs of Argylia radiata, an extremophile plant from Chilean Atacama desert
    (2020) Morales Tapia, Pablo Andrés; Cabrera Barjas, G.; Giordano Villatoro, Ady
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    Relation between composition, antioxidant and antibacterial activities and botanical origin of multi-floral bee pollen.
    (2017) Velásquez, P.; Rodríguez, K.; Retamal, M.; Giordano Villatoro, Ady; Valenzuela Roediger, Loreto Margarita; Montenegro Rizzardini, Gloria
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    Toward the cholinesterase inhibition potential of TADDOL derivatives: Seminal biological and computational studies
    (2022) Constantino, Andrea R.; Charbe, Nitin Bharat; Duarte, Yorley; Gutierrez, Margarita; Giordano Villatoro, Ady; Prasher, Parteek; Dua, Kamal; Mandolesi, Sandra; Zacconi, Flavia C. M.
    Alzheimer's disease (AD) is a degenerative neurological disease characterized by gradual loss of cognitive skills and memory. The exact pathogenesis involved still remains unrevealed, but several studies indicate the involvement of an array of different enzymes, underlining the multifactorial character of the disease. Inhibition of these enzymes is therefore a powerful approach in the development of AD treatments, with promising candidates, including acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and monoamine oxidase. Interestingly, AChE is the target of a major pesticide family (organophosphates), with several reports indicating an intersection between the pesticide's activity and AD. In this study, various TADDOL derivatives were synthesized and their in vitro activities as AChE/BuChE inhibitors as well as their antioxidant activities were studied. Molecular modeling studies revealed the capability of TADDOL derivatives to bind to AChE and induce inhibition, especially compounds 2b and 3c furnishing IC50 values of 36.78 +/- 8.97 and 59.23 +/- 5.31 mu M, respectively. Experimental biological activities and molecular modeling studies clearly demonstrate that TADDOL derivatives with specific stereochemistry have an interesting potential for the design of potent AChE inhibitors. The encouraging results for compounds 2b and 3c indicate them as promising scaffolds for selective and potent AChE inhibitors.