Browsing by Author "Gaskell, J"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora
    (2001) Tello, M; Seelenfreund, D; Lobos, S; Gaskell, J; Cullen, D; Vicuña, R
    Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of sport: forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB. their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of neu genes. (C) 2001 Federation of European Microbiological Societies. published by Elsevier Science B.V. All rights reserved.
  • No Thumbnail Available
    Item
    lip-like genes in Phanerochaete sordida, and Ceriporiopsis subvermispora, white rot fungi with no detectable lignin peroxidase activity
    (1996) Rajakumar, S; Gaskell, J; Cullen, D; Lobos, S; Karahanian, E; Vicuna, R
    Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described LiP genes. Using reverse transcription-coupled PCR a LiP-like cDNA clone was amplified from P. sordida RNA. In contrast, no evidence was obtained for transcription of C. subvermispora LiP genes.