Browsing by Author "Garg, A."

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    Absence of AGPAT2 impairs brown adipogenesis, increases IFN stimulated gene expression and alters mitochondrial morphology
    (2020) Tapia Ossa, Pablo José; Figueroa Toledo, Ana María; Eisner Sagüés, Verónica Raquel; González Hódar, Lila Alejandra; Robledo Plaza, Fermín Alberto; Agarwal, AK; Garg, A.; Cortés Mora, Víctor Antonio
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    Decision-Making about Healthcare Related Tests and Diagnostic Strategies: User Testing of GRADE Evidence Tables
    (2015) Mustafa, R.; Wiercioch, W.; Santesso, N.; Cheung, A.; Prediger, B.; Baldeh, T.; Carrasco, A.; Brignardello, R.; Neumann Burotto, Gonzalo Ignacio; Bossuyt, P.; Garg, A.; Lelgemann, M.; Buhler, D.; Brozek, J.; Schunemann, H.
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    Decreased caveolae in AGPAT2 lacking adipocytes is independent of changes in cholesterol or sphingolipid levels: A whole cell and plasma membrane lipidomic analysis of adipogenesis
    (2021) González-Hódar, L.; McDonald, J.G.; Vale, G.; Thompson, B.M.; Figueroa, A.-M.; Tapia, P.J.; Robledo, F.; Agarwal, A.K.; Garg, A.; Horton, J.D.; Cortés, V.
    Background Adipocytes from lipodystrophic Agpat2−/− mice have impaired adipogenesis and fewer caveolae. Herein, we examined whether these defects are associated with changes in lipid composition or abnormal levels of caveolae-associated proteins. Lipidome changes were quantified in differentiated Agpat2−/− adipocytes to identify lipids with potential adipogenic roles. Methods Agpat2−/− and wild type brown preadipocytes were differentiated in vitro. Plasma membrane was purified by ultracentrifugation. Number of caveolae and caveolae-associated proteins, as well as sterol, sphingolipid, and phospholipid lipidome were determined across differentiation. Results Differentiated Agpat2−/− adipocytes had decreased caveolae number but conserved insulin signaling. Caveolin-1 and cavin-1 levels were equivalent between Agpat2−/− and wild type adipocytes. No differences in PM cholesterol and sphingolipids abundance were detected between genotypes. Levels of phosphatidylserine at day 10 of differentiation were increased in Agpat2−/− adipocytes. Wild type adipocytes had increased whole cell triglyceride, diacylglycerol, phosphatidylglycerol, phosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, and trihexosyl ceramide, and decreased 24,25-dihydrolanosterol and sitosterol, as a result of adipogenic differentiation. By contrast, adipogenesis did not modify whole cell neutral lipids but increased lysophosphatidylcholine, sphingomyelin, and trihexosyl ceramide levels in Agpat2−/− adipocytes. Unexpectedly, adipogenesis decreased PM levels of main phospholipids in both genotypes. Conclusion In Agpat2−/− adipocytes, decreased caveolae is not associated with changes in PM cholesterol nor sphingolipid levels; however, increased PM phosphatidylserine content may be implicated. Abnormal lipid composition is associated with the adipogenic abnormalities of Agpat2 −/− adipocytes but does not prevent insulin signaling.
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    HIGH-AMPLITUDE delta-SCUTIS IN THE LARGE MAGELLANIC CLOUD
    (2010) Garg, A.; Clocchiatti, Alejandro; Minniti, D.
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    HIGH-AMPLITUDE δ-SCUTIS IN THE LARGE MAGELLANIC CLOUD
    (2010) Garg, A.; Cook, K. H.; Nikolaev, S.; Huber, M. E.; Rest, A.; Becker, A. C.; Challis, P.; Clocchiatti, A.; Miknaitis, G.; Minniti, D.; Morelli, L.; Olsen, K.; Prieto, J. L.; Suntzeff, N. B.; Welch, D. L.; Wood-Vasey, W. M.
    We present 2323 high-amplitude delta-Scuti (HADS) candidates discovered in the Large Magellanic Cloud by the SuperMACHO survey (Rest et al.). Frequency analyses of these candidates reveal that several are multimode pulsators, including 119 whose largest amplitude of pulsation is in the fundamental (F) mode and 19 whose largest amplitude of pulsation is in the first overtone (FO) mode. Using Fourier decomposition of the HADS light curves, we find that the period-luminosity (PL) relation defined by the FO pulsators does not show a clear separation from the PL relation defined by the F pulsators. This differs from other instability strip pulsators such as type c RR Lyrae. We also present evidence for a larger amplitude, subluminous population of HADS similar to that observed in Fornax.