Browsing by Author "Fuentealba, Claudia"

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    Cell wall disassembly, metabolome and transcriptome analysis in sweet cherry fruit with induced surface pitting
    (2023) Ponce, Excequel; Nunez-Lillo, Gerardo; Bravo, Camila; Vidal, Juan; -Reyes, Patricio Tapia; Meneses, Claudio; Pedreschi, Romina; Fuentealba, Claudia
    Surface pitting is the main quality problem that develops during prolonged storage of sweet cherries. It appears as one or more depressions on the surface of the fruit and is associated with the collapse of cells under the skin of the fruit. However, this physiological disorder is not shown in all cultivars with the same intensity. This research aims to analyze the cell wall disassembly of two cultivars displaying contrasting susceptibility to damage after pitting induction. In addition, we evaluated the metabolomic and transcriptomic changes in sweet cherries during cold storage. Our results show that damage to 'Sweetheart' fruit was more severe than damage to 'Bing' fruit. No differences were observed in the cell wall composition between nonpitted and pitted cherries; however, the varietal differences during cold storage were the most significant. The resistant cultivar 'Bing' showed a longer sidechain of RG-I; instead, 'Sweetheart' PME and PG activity was more marked at the beginning of cold storage. The metabolomics analysis revealed several compounds related to the abiotic stress response, such as 3O-coumaroyl-D-quinic acid, chlorogenic acid, GABA and beta-sitosterol. Furthermore, transcriptomics showed a higher expression of stress-related hormones in the susceptible cultivar and cell wall remodeling-related genes in the resistant cultivar. In conclusion, the contrasting susceptivity to surface pitting in sweet cherries can be attributed to the varietal response to cold storage rather than the mechanical stress of pitting induction.
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    Proteomics analysis reveals new insights into surface pitting of sweet cherry cultivars displaying contrasting susceptibility
    (2022) Nunez-Lillo, Gerardo; Ponce, Excequel; Alvaro, Juan E.; Campos, David; Meneses, Claudio; Campos-Vargas, Reinaldo; Carpentier, Sebastien; Fuentealba, Claudia; Pedreschi, Romina
    Surface pitting in sweet cherry (Prunus avium L.) is characterised by depressions development on the skin surface. Pitting damage happens during harvest and postharvest handling and develops during cold storage. This study compared two cultivars with contrasting susceptibility to pitting (Kordia: tolerant; Sweetheart: susceptible) using a LC-MS/MS proteomic approach to identify key metabolic and signalling pathways related to this disorder during ripening and postharvest storage. The variability observed in the principal component analysis was driven by the cultivars suggesting that surface pitting susceptibility is triggered by the inherent differences between cultivars. Proteins involved in anthocyanin biosynthesis were more abundant in Kordia. Moreover, CCR (cinnamoyl-CoA reductase) and FLS (flavonol synthase) proteins showed higher abundance in Sweetheart. An overexpression in enzymes related to the synthesis of abscisic acid (ABA), jasmonic acid (JA) and ethylene were found in Kordia. A higher abundance of sucrose synthase (SUS), UDP-glycosyltransferases (UDP-GT) and polygalacturonase-inhibiting proteins (PGIP) were observed in Kordia, while a higher content of invertase (INV) was observed in Sweetheart. The overexpression of the studied pathways suggests that tolerance to surface pitting could be correlated to one or more of these factors, and susceptibility might be given by the inherent differences in the metabolic processes of each cultivar.