Browsing by Author "Ferres Garrido, Marcela Viviana"
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- ItemA simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR(2020) Wozniak Banchero, Aniela; Cerda Rojas, Ariel Patricio; Lamig Giannini, Liliana Andrea; Solari Gajardo, Sandra; Guzmán Durán, Ana María; Riveras Hernández, Eleodoro Javier; Ferres Garrido, Marcela Viviana; Gutiérrez Ilabaca, Rodrigo Antonio; García Cañete, Patricia; Ibarra Henriquez, C.; Sebastian, V.; Armijo, G.; Lamig, L.; Miranda, C.; Lagos, M.; Quiroga, T.; Hitschfeld, S.
- ItemA Single-Nucleotide Polymorphism of (V3) Integrin Is Associated with the Andes Virus Infection Susceptibility(2019) Martinez Valdebenito, Constanza Pamela; Angulo Troncoso Jenniffer Alexandra; Le Corre Pérez, Monique Nicole; Marco Caceres, Claudia Alejandra; Vial, Cecilia; Miquel Poblete, Juan Francisco; Cerda Lorca, Jaime Rodrigo; Mertz, Gregory; Vial, Pablo; Lopez Lastra, Marcelo Andres; Ferres Garrido, Marcela Viviana
- ItemAporte de la biología molecular en el diagnóstico de infecciones respiratorias agudas(2016) Budnik Ojeda, Isolda Cecilia; Ferres Garrido, Marcela Viviana; Pardo Tarrago, Trinidad; Edwards Tagle, Javiera; Labarca Trucios, Gonzalo Patricio; Reyes Zaldivar, Felipe Tomas; Martínez Valdebenito, Constanza Pamela; Montecinos Perret, Luisa Paola; Perret Pérez, Cecilia
- ItemCaracterísticas clínicas de la enfermedad respiratoria causada por Mycoplasma pneumoniae en niños hospitalizados(SOC CHILENA INFECTOLOGIA, 2009) Paul Delfau, María de los Ángeles; Vega Briceño, Luis Enrique; Potin Santander, Marcela Patricia; Ferres Garrido, Marcela Viviana; Pulgar B., Dahiana; García Bruce, Cristián Jorge; Holmgren Palmen, Nils Linus Anders; Sánchez Díaz, Ignacio
- ItemClinical and epidemiological manifestations of parainfluenza infection in hospitalized children(SOC CHILENA INFECTOLOGIA, 2007) Vega Briceño, Luis Enrique; Pulgar B., Dahiana; Potin Santander, Marcela Patricia; Ferres Garrido, Marcela Viviana; Sánchez Díaz, Ignacio
- ItemClinical-epidemiological analysis of infections in pediatric patients with liver and heart trasplants(SOC CHILENA INFECTOLOGIA, 2024) Jung Montanares, Francisca Andrea; Astudillo Paredes, Patricio Andrés; Vrsalovic Papic, Katica Ivanka; Rosales Alfaro, Valentina José; Marinovic, Nicole L'Huillier; Vizcaya Altamirano, María Cecilia; Ferres Garrido, Marcela Viviana; Urcelay Montecinos, Gonzalo Eduardo; Gana Ansaldo, Juan Cristobál; Abarca Villaseca, KatiaBackground: Liver and heart transplantation represents the definitive treatment for end-stage failure of these organs. In recent years, an increasing number of pediatric patients have undergone these procedures, which are associated with various complications. Among them, infections stand out as one of the main causes of morbidity and mortality. Aim: To describe the infections that affect pediatric recipients of liver and heart transplants (Tx.). Methods: A characterization of the type and timing of infections identified in 31 children who underwent heart transplantation and 27 who underwent liver transplantation at a tertiary university hospital since 2002 (cardiac Tx) and 2009 (hepatic Tx), both to 2022 is presented. Results: Biliary atresia and dilated cardiomyopathy were the main reasons for liver and heart transplantation, respectively. Bacterial infections predominated during the first month after these surgical procedures, while viral infections predominated later. Bacterial infections in general, and bacteriemia were more common in liver Tx. recipients. Cytomegalovirus (CMV) and Epstein-Barr virus, mainly in the form of reactivations, were the most frequently identified viruses, with a higher frequency of CMV in liver Tx. recipients. One patient from each group died in a late period due to bacterial sepsis, without a specific agent identified. Conclusion: The preponderance of infections due to latent viral agents, such as CMV, in pediatric liver or heart transplant recipients, reveals the need to optimize the management of these infections in this growing population in Chile.
- ItemConsenso sobre riesgo de complicaciones infecciosas en pacientes usuarios de medicamentos biológicos seleccionados. Parte II: Guía clínica chilena de Prevención de Infecciones Asociadas al Uso de Terapias Biológicas (PREVITEB)(2019) Cerón Araya, Inés María; Vizcaya Altamirano, María Cecilia; Gambra, Pilar; Ferres Garrido, Marcela Viviana; Bidart, Teresa; Lépez Quizhpi,Tania Lorena; Acuña, María Paz; Álvarez, Ana María; Zubieta, Marcela; Durán, Luisa; Rabagliati Borie, Ricardo MiguelThe use of biological therapies has meant a great improvement in the management of several conditions like autoimmune, neoplastic or others diseases. Although its use has implied significant improvements in the prognosis of these diseases, it is not exempt from complications: infectious diseases as one of them. The objective of this consensus was to evaluate, from an infectious viewpoint, the safeness of the most frequently used biological therapies and give recommendations for the prevention of infections in patients treated with these drugs. These recommendations were based on the highest quality evidence available for the selected biologics. The consensus counts of 2 manuscripts. This second part is a guideline that details these recommendations through screening strategies, prophylactic therapies and vaccines indications for bacterial, mycobacterial, viral, fungal and parasitic infections, both for adults and children.
- ItemHantavirosis: Clinical and epidemiological characteristics of pediatric patients in Chile(Sociedad Chilena de Infectologia, 2010) Ferres Garrido, Marcela Viviana; Sandoval Carmona, Carmen Alejandra; Delgado B, Iris; Sotomayor P, Viviana; Olea N, AndreaBackground: The first cases of hantavirus cardiopulmonary syndrome in children were described in the United States and represented 8% of the total reported cases, in Chile the proportion of pediatric cases represents 15% of all national cases. Aim: To describe the epidemiology and clinical course of 82 children reported to the Chilean Ministry of Health up to 2007 and to characterize more extensively a subgroup of 24 children whose detailed clinical data were available. Results: Forty patients were under 10 years old. Seventeen (17/82) of 82 cases (20.7%) presented in the context of a family cluster. Ninety eight percent of cases (80/82) occurred among individuals living in rural areas and 66% during summer months). The overall fatality rate was 36.6%. Fever (93%), respiratory distress (75%) and gastrointestinal symptoms (75%) were the most frequent symptoms encountered in the 28 children studied more extensively. Abnormal blood coagulation test were significantly associated with death while an increased hematocrit was associated with severe cases (hemodynamic unstability). Conclusion: An early diagnosis should favor early onset of aggressive treatment that could potentially save lives. Increasing knowledge on the clinical presentation of the disease in children should improve early clinical diagnosis among health care professionals.
- ItemHuman metapneumovirus as hospitalization cause in children under 3 years old with acute respiratory infections during 2004(2007) Prado Sanhueza, Maria Alejandra; Perret Pérez, Cecilia; Montecinos Perret, Luisa Paola; Veloz B., A.; Le Corre Pérez, Monique Nicole; Habash A., L.; Potin Santander, Marcela Patricia; Abarca Villaseca, Katia; Ferres Garrido, Marcela VivianaMetapneumovirus humano (MPVh) fue detectado entre julio y noviembre en 15 de 123 niños bajo 3 años de edad hospitalizados por infección respiratoria aguda (12%). Las muestras fueron estudiadas mediante técnicas de biología molecular (RPC-TR de muestra de hisopado nasofaríngeo y/o de sobrenadante de cultivo). El 67% de los niños hospitalizados con MPVh tenían menos de 1 año de edad, todos ellos presentaron tos y fiebre y el principal motivo de hospitalización fue el requerimiento de oxígeno en 73% de los casos. Si bien un tercio de los pacientes tenía patología previa, su evolución clínica no fue diferente respecto de los niños previamente sanos. El patrón radiológico mostró aumento de la trama intersticial, con focos de consolidación en 6 casos (40%). El diagnóstico más frecuente fue síndrome bronquial obstructivo o bronquiolitis, asociado o no a neumonía. Destaca la necesidad de un método de diagnóstico rápido para optimizar el diagnóstico diferencial, manejo y control de infecciones en estos pacientes.
- ItemImportance of images and etiological diagnosis of central nervous system envolvement in immunocompromised patient(2010) Ferres Garrido, Marcela Viviana; Bidart H, Teresa; Zubieta A, MarcelaComunicamos el caso de un adolescente de 16 años, con una leucemia mieloide aguda y una grave inmunosupresión secundaria a su enfermedad y el tratamiento. Precozmente post-quimioterapia desarrolló síntomas y signos compatibles con una enfermedad fúngica invasora (EFI). Inicialmente se afectaron sus pulmones y a continuación el SNC con hipertensión intracraneal. Los exámenes de laboratorio indicaron una neutropenia prolongada y evidencias indirectas de una infección por Aspergillus sp mediante la medición sucesiva de galactomanano positivo en sangre y un segundo valor en ascenso. Con este caso enfatizamos la gran importancia que tienen las EFI en pacientes inmunocomprometidos, particularmente sobre el SNC. Ellas representan una emergencia médica que requiere de una confín-nación microbiológica y el inicio temprano de terapia anti-fúngica empírica. Debe destacarse la importancia que tienen las neuro-imágenes en la correcta identificación del número de lesiones, su localization y tamaño. La RM, si está disponible, debiera ser la elección, para una mejor definición, por sobre el uso de la TAC. Igualmente, debiera discutirse la indicación de biopsia cerebral cuando todos los métodos no invasores han fracasado en precisar la etiología. La EFI del SNC es de tratamiento médico, debiéndose considerar el drenaje quirúrgico de las lesiones con más de 2 cm de diámetro o aquellas que ejercen efecto de masa o, finalmente, cuando ha fracasado el manejo con anti-fúngic
- ItemInfection with SARS-CoV-2 variant Gamma (P.1) in Chile increased ICU admission risk three to five-fold(2023) Sauré, Denis; Rizzo, Alessandro; Neira, Ignasi; Goic, Marcel; O’Ryan, Miguel; Torres, Juan P.; Bruhn, Alejandro; Ferres Garrido, Marcela Viviana; Angulo Troncoso, Jenniffer Alexandra; Vera Alarcon, Maria Magdalena; Basso, Leonardo J.The 2021 wave of SARS-CoV-2 infection in Chile was characterized by an explosive increase in ICU admissions, which disproportionately affected individuals younger than 60 years. This second wave was also accompanied by an explosive increase in Gamma (P.1) variant detections and the massive vaccine rollout. We unveil the role the Gamma variant played in stressing the use of critical care, by developing and calibrating a queueing model that uses data on new onset cases and actual ICU occupancy, symptom’s onset to ICU admission interval, ICU length-of-stay, genomic surveillance, and vaccine effectiveness. Our model shows that infection with the Gamma (P.1) variant led to a 3.5–4.7-fold increase in ICU admission for people younger than 60 years. This situation occurred on top of the already reported higher infection rate of the Gamma variant. Importantly, our results also strongly suggest that the vaccines used in Chile (inactivated mostly, but also an mRNA), were able to curb Gamma variant ICU admission over infections.
- ItemLas lecciones de Epuyén: a un año del brote de virus Hanta(SOC MEDICA SANTIAGO, 2020) Dominguez, Gaspar; Gonzalez Coloma, Francisco; Oyarzun, Daniela; Ferres Garrido, Marcela Viviana; Martinez Valdebenito, Constanza Pamela; Henríquez Franco, Carolina Andrea; Lavarra, Enzo
- ItemPREDICTING HANTAVIRUS RISK IN CHILE(AMER SOC TROP MED & HYGIENE, 2008) Glass, Gregory E.; Marquet Iturriaga Pablo Angel; Palma Troncoso Rodrigo Eduardo; Barria, Ivan; Yates, Terry L.; Vial, Pablo A.; Ferres Garrido, Marcela Viviana; Mertz, Gregory J.
- ItemPresence of Bartonella henselae in cats, in Chile(Sociedad Medica de Santiago, 2005) Ferres Garrido, Marcela Viviana; Abarca Villaseca, Katia; Godoy M., Paula; Chanqueo C., Leonardo; Garcia Canete, Patricia Del Carmen; Palavecino, Elizabeth; Vial C., Pablo A.; Gabriela Méndez R.; Valdés O., Alicia; Ernst M., Santiago; Thibaut L., Julio; Koberg J.The availability of a serologic test for cat scratch disease in humans has allowed the diagnosis of an increasing number of cases of this disease in Chile. Aim: To perform a serological survey for Bartonella henselae among cats in Chile. Material and methods: Blood samples from 187 cats living in three Chilean cities were obtained. IgG antibodies againts Bartonella henselae were measured using indirect immunofluorescence. Blood cultures were done in 60 samples. The presence of Bartonella henselae in positive cultures was confirmed by restriction fragment length polymorphism polimerase chain reaction (RFLP-PCR). Results: The general prevalence of IgG antibodies against Bartonella henselae was 85.6%. No differences in this prevalence were found among cats younger or older than 1 year, or those infested or not infested with fleas. However domestic cats had a lower prevalence when compared with stray cats (73 and 90% respectively, p <0.01). Bartonella henselae was isolated in 41%of blood cultures. All the isolated were confirmed as Bartonella henselae by KFLP-PCR Conclusions: This study found an important reservoir of Bartonella henselae in Chilean cats and therefore a high risk of exposure in humans who have contact with them (Rev Méd Chile 2005; 133:1465-71).
- ItemPrevalencia de anticuerpos contra Bartonella henselae en niños, en adolescentes y en una población de riesgo ocupacional en Chile(2006) Ferres Garrido, Marcela Viviana; Abarca Villaseca, Katia; Prado, Priscilla; Montecinos Perret Luisa Paola; Navarrete C, Maritza; Vial C, Pablo A.Background: Bartonella henselae is the causative agent of cat-scratch disease. Aim: To study the seroepidemiology of Bartonella henselae in healthy Chilean children and in a population with occupational risk. Material and methods: Serum IgG antibodies were determined by indirect fluorescence technique in 181 children and adolescents and in 107 technical and professional workers involved in the care of cats. Samples with titers equal to or greater than 64 were considered positive. Results: Twenty four (13.3%) children and 11 (10.3%) occupational risk subjects were seropositive. No significant differences by age and gender were observed. Conclusions: Assuming that seroprevalence indicates level of exposure to Bartonella henselae, these results suggest that this infection is endemic in Chile and, for this reason, the best antibody titer to diagnose acute cat-scratch disease should be higher than the figure recommended by the Centers for Disease Control in the in United States.
- ItemReduced Immune Response to Inactivated Severe Acute Respiratory Syndrome Coronavirus 2 Vaccine in a Cohort of Immunocompromised Patients in Chile(Oxford University Press for the Infectious Diseases Society of America, 2022) Balcells Marty, María Elvira; Le Corre Pérez, Monique Nicole; Durán Santa Cruz, Josefina Gracia; Ceballos Valdivielso, María Elena Andrea; Vizcaya Altamirano, María Cecilia; Mondaca Contreras, Sebastián Patricio; Dib Marambio, Martin Javier; Rabagliati Borie, Ricardo Miguel; Sarmiento Maldonado, Mauricio; Burgos Cañete, Paula Isabel; Espinoza Sepúlveda, Manuel Antonio; Ferres Garrido, Marcela Viviana; Martínez Valdebenito, Constanza Pamela; Ruiz-Tagle Seguel, Cinthya Grace; Ortiz Koh, Catalina Alejandra; Ross Pérez, Patricio Daniel; Budnik Bitran, Sigall; Solari Gajardo, Sandra; Vizcaya Vergara, María De Los Ángeles; Lembach, Hanns; Berríos Rojas, Roslye; Melo González, Felipe; Rios Raggio, Mariana; Kalergis Parra, Alexis Mikes; Bueno Ramírez, Susan Marcela; Nervi Nattero, BrunoBackground Inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines have been widely implemented in low- and middle-income countries. However, immunogenicity in immunocompromised patients has not been established. Herein, we aimed to evaluate immune response to CoronaVac vaccine in these patients. Methods This prospective cohort study included 193 participants with 5 different immunocompromising conditions and 67 controls, receiving 2 doses of CoronaVac 8-12 weeks before enrollment. The study was conducted between May and August 2021, at Red de Salud UC-CHRISTUS, Santiago, Chile. Neutralizing antibody (NAb) positivity, total anti-SARS-CoV-2 immunoglobulin G antibody (TAb) concentrations, and T-cell responses were determined. Results NAb positivity and median neutralizing activity were 83.1% and 51.2% for the control group versus 20.6% and 5.7% (both P < .001) in the solid organ transplant group, 41.5% and 19.2% (both P < .0001) in the autoimmune rheumatic diseases group, 43.3% (P < .001) and 21.4% (PP = .001) in the cancer with solid tumors group, 45.5% and 28.7% (both P < .001) in the human immunodeficiency virus (HIV) infection group, 64.3% and 56.6% (both differences not significant) in the hematopoietic stem cell transplant group, respectively. TAb seropositivity was also lower for the solid organ transplant (20.6%; P < .0001), rheumatic diseases (61%; P < .001), and HIV groups (70.9%; P = .003), compared with the control group (92.3%). On the other hand, the number of interferon gamma spot-forming T cells specific for SARS-CoV-2 tended to be lower in all immunocompromising conditions but did not differ significantly between groups. Conclusions Diverse immunocompromising conditions markedly reduce the humoral response to CoronaVac vaccine. These findings suggest that a boosting vaccination strategy should be considered in these vulnerable patients.
- ItemSupport of the laboratory of microbiology and pathological anatomy in the diagnosis and management of infections in cancer patients and transplantation of hematopoietic stem cell transplant receptors(2019) Ferres Garrido, Marcela Viviana; Lafourcade, Monica; Gambra, Pilar; Ceron Araya, Ines Maria; Paya, Ernesto; Oddo Benavides, Carlos DavidThe confrontation of the differential and etiological diagnosis of the infectious diseases of cancer patients, including hematopoietic stem cells transplant (HSCT) recipients, must correspond to an informed, timely decision that directly affects medical behavior that determines a better survival and quality of life for patients. The main goal of this work was to contribute to the management of these patients developing a useful tool for the clinician to make these decisions. For that, infections were grouped by compromised systems, differentiating the possible etiological agents in bacteria, viruses, fungi and parasites, highlighting the relevant diagnostic tests, mentioning the recommended techniques together with the optimal sample type for proper processing. In addition, under each group of techniques we added the item "level of requirement" to suggest what, in the opinion of the authors and the existing evidence, must be mandatory to have at local level or can be derivable to another laboratory.
- ItemSusceptibilidad genética a hantavirus Andes: Asociación entre la expresión clínica de la infección y alelos del sistema HLA en pacientes chilenos(2007) Ferrer C., Pablo; Vial Claro, Pablo Agustín; Ferres Garrido, Marcela Viviana; Godoy Mayorga, Paula Carolina; Cuiza Vásquez, Analía; Marco Caceres Claudia Alejandra; Castillo H., Constanza; Umana C., Maria Elena; Rothhammer E., Francisco; Llop R., ElenaAndes hantavirus (ANDV) infection in Chile has a variable clinical expression, and infected individuals may present with different grades of disease severity. This study aimed to determine if clinical expression of ANDV infection in Chilean patients is associated with the HLA system. HLA alleles A, B, DRB1 and DQB1, were studied in two groups of patients with confirmed ANDV infection: 41 patients with a mild disease course (without respiratory failure and cardiovascular shock) and 46 patients with a severe disease course (with respiratory failure and shock). Molecular typing of HLA system was performed by SSP-PCR. The HLA-DRB1*15 allele, was significantly more common in the group of patients with mild disease (p = 0,007) and thus for possibly associated with a protective effect against ANDV infection. Conversely, HLA-B*08 was more common in patients with severe disease (p = 0,06). Although the association was marginally significant, allele HLA-B*08 may be linked to an increased susceptibility to the severe clinical course of HCPS by ANDV.