Browsing by Author "Faunes Quinteros, Fernando Emerson"

Now showing 1 - 8 of 8
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    A role for Lin-28 in growth and metamorphosis in Drosophila melanogaster
    (2018) Gonzalez-Itier, Sergio; Contreras, Esteban G.; Larraín Correa, Juan Agustín; Glavic, Alvaro; Faunes Quinteros, Fernando Emerson
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    Characterization of small RNAs in X. tropicalis gastrulae
    (2012) Faunes Quinteros, Fernando Emerson; Almonacid, L. I.; Melo Ledermann, Francisco Javier; Larraín Correa, Juan Agustín
    Here, we report and characterize deep sequencing data and bioinformatics analysis of small RNAs from X. tropicalis gastrula. A total of 17,553,124 reads with perfect match to the genome derived from 2,616,053 unique sequences were identified. Seventy-seven percent of theses sequences were not found in previous reports from X. tropicalis oocytes and somatic tissues. Bioinformatics analyses indicate that a large fraction of the small RNAs are PIWI-interacting RNAs. Up to 23.9% of small RNAs mapped to transposable elements and 27% to genic regions. Most of the abundant transposon-derived small RNAs are found in oocyte and gastrula libraries, suggesting that transposons also need to be silenced during early embryonic development. Importantly, novel clusters of piRNAs whose expression is activated after zygotic transcription begins were identified in the genome of X. tropicalis. Additionally, miRNAs were also identified and many of them are not present in oocytes, suggesting that miRNA expression is stage-specific. To the best of our knowledge, this is the first high throughput data release and bioinformatics characterization of small RNAs during Xenopus early embryonic development. genesis 50:572–58, 2012. © 2012 Wiley Periodicals, Inc.
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    Conservation in the involvement of heterochronic genes and hormones during developmental transitions
    (2016) Faunes Quinteros, Fernando Emerson; Larraín Correa, Juan Agustín
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    Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression
    (2009) Faunes Quinteros, Fernando Emerson; Vergara Saavedra, Ismael Antonio; Melo Ledermann, Francisco Javier; Larraín Correa, Juan Agustín
    Background: Recent evidence from global studies of gene expression indicates that transcriptomes are more complex than expected. Xenopus has been typically used as a model organism to study early embryonic development, particularly dorso-ventral patterning. In order to identify novel transcripts involved in dorso-ventral patterning, we compared dorsal and ventral transcriptomes of Xenopus tropicalis at the gastrula stage using serial analysis of gene expression ( SAGE).
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    Overexpression of Lin28a delays Xenopus metamorphosis and down-regulates albumin independently of its translational regulation domain
    (2019) Gundermann, D.G.; Martinez, J.; De Kervor, G.; González Pinto, Karina; Larraín Correa, Juan Agustín; Faunes Quinteros, Fernando Emerson
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    The heterochronic gene Lin28 regulates amphibian metamorphosis throughdisturbance of thyroid hormone function
    (2017) Faunes Quinteros, Fernando Emerson; Gundermann, Daniel G.; Muñoz, Rosana; Bruno, Renzo; Larraín Correa, Juan Agustín
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    Transcriptomics using next generation sequencing technologies
    (2012) Lee Liu, D.; Almonacid, L. I.; Faunes Quinteros, Fernando Emerson; Melo Ledermann, Francisco Javier; Larraín Correa, Juan Agustín
    Next generation sequencing technologies may now be applied to the study of transcriptomics. RNA-Seq or RNA sequencing employs high-throughput sequencing of complementary DNA fragments delivering a transcriptional profile. In this chapter, we aim to provide a starting point for Xenopus researchers planning on starting an RNA-Seq transcriptomics study. We begin by providing a section on template isolation and library preparation. The next section comprises the main bioinformatics procedures that need to be performed for raw data processing, normalization, and differential gene expression. Finally, we have included a section on studying deep sequencing results in Xenopus, which offers general guidance as to what can be done in this model.
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    Transcriptomics using next generation sequencing technologies
    (2012) Lee Liu, Dasfne Nicole.; Faunes Quinteros, Fernando Emerson; Melo Ledermann, Francisco Javier