Browsing by Author "FUENZALIDA, I"

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    HAMSTER OOCYTE FERTILIZABILITY AFTER 4-DEGREES-C STORAGE
    (1986) BARROS, C; HERRERA, E; FUENZALIDA, I; ARGUELLO, B
    The purpose of the present work was to study the feasibility of using hamster oocytes stored at 4.degree. C in M-2 culture medium for 24 and 48 hours in the evaluation of human sperm fertilizability. A total of 1,394 oocytes were stored for 24 hours and 1,234 were stored for 48 hours. After the storage period all the oocytes were stained with fluorescein diacetate, proving the physical integrity of the egg plasma membrane. Twenty-five and 22 semen samples were used to compare their ability to penetrate freshly ovulated and 24- and 48-hour-stored hamster oocytes. Freshly ovulated and 24-hour-stored oocytes were penetrated at percentages that in more than 95% of the cases showed no significant differences. The same experiments carried out with oocytes stored for 48 hours showed that in 75% of the cases no significant differences were found. The use of oocytes preserved at 4.degree. C when large numbers of semen samples are to be tested for fertilizability is recommended.
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    HUMAN SPERM-CERVICAL MUCUS INTERACTION AND THE ABILITY OF SPERMATOZOA TO FUSE WITH ZONA-FREE HAMSTER OOCYTES
    (1988) BARROS, C; JEDLICKI, A; FUENZALIDA, I; HERRERA, E; ARGUELLO, B; VIGIL, P; VILLASECA, P; LEONTIC, E
    Samples of semen and cervical mucus were provided by 18 couples. Cervical mucus was obtained for each day possible and stored at 4.degree.C until all the samples were collected. Flat capillary tubes were loaded with the mucous samples and spermatozoa from the husband''s semen sample were allowed to migrate through the cervical mucus (3 cm column) into culture medium. The spermatozoa recovered after migration through cervical mucus were assayed in vitro with zona-free hamster oocytes. Control experiments were carried out using spermatozoa from the same semen sample but prepared by the swimming-up technique. Altogether, 557 eggs in the control group and 1236 eggs in the experimental group were analysed, and the results demonstrated that the % of sperm penetration, the mean number of sperm decondensations per penetrated egg and the mean number of spermatozoa adhering per egg all had higher values (P < 0.05) for the control samples than for the experimental samples. We suggest that cervical mucus modifies human spermatozoa, as measured by their interaction with zona-free hamster oocytes.