Browsing by Author "Enrione, J."

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    Cold-adaptation of a methacrylamide gelatin towards the expansion of the biomaterial toolbox for specialized functionalities in tissue engineering
    (2019) Zaupa, A.; Byres, N.; Dal Zovo, C.; Acevedo, C.A.; Angelopoulos, I.; Terrazaa, C.; Nestle, N.; Abarzua-Illanes, P.N.; Quero, F.; Zacconi, Flavia C. M.; Diaz-Calderon, P.; Olguin, Y.; Akentjew, T.L.; Wilkens, C.A.; Padilla, C.; Pino-Lagos, K.; Blaker, U.J.; Khoury, M.; Enrione, J.; Acevedo, J.P.
    Tissue regeneration is witnessing a significant surge in advanced medicine. It requires the interaction of scaffolds with different cell types for efficient tissue formation post-implantation. The presence of tissue subtypes in more complex organs demands the co-existence of different biomaterials showing different hydrolysis rate for specialized cell-dependent remodeling. To expand the available toolbox of biomaterials with sufficient mechanical strength and variable rate of enzymatic degradation, a cold-adapted methacrylamide gelatin was developed from salmon skin. Compared with mammalian methacrylamide gelatin (GeIMA), hydrogels derived from salmon GelMA displayed similar mechanical properties than the former. Nevertheless, salmon gelatin and salmon Ge1MA-derived hydrogels presented characteristics common of cold-adaptation, such as reduced activation energy for collagenase, increased enzymatic hydrolysis turnover of hydrogels, increased interconnected poly-peptides molecular mobility and lower physical gelation capability. These properties resulted in increased cell remodeling rate in vitro and in vivo, proving the potential and biological tolerance of this mechanically adequate cold-adapted biomaterial as alternative scaffold subtypes with improved cell invasion and tissue fusion capacity.
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    Food Hydrocolloid Edible Films and Coatings
    (Nova Publishers, 2010) Aguilera Radic, José Miguel; Pedreschi Plasencia, Franco Wilfredo; Acevedo, C.; Enrione, J.; Osorio, F.; Skurtys, O.; Hollingworth, Clarence S.
    In this chapter book, we present and discuss some aspects of hydrocolloid edible films and coating. The film-forming materials is presented and the physical properties (e.g. thickness, mechanical, optical and thermodynamic properties, water and gases barrier) of the film and coating are examined in detail. The main techniques used to form edible coatings as spray systems or immersion procedures are discussed as well as the solvent casting and the extrusion processes for the edible films. The main disadvantage of these techniques is the lost of quality of the edible coatings and films since there is no control over the shape, size and size distribution of the dispersed elements (e.g. additives, ingredients, etc.) in the support structure matrix is poor. Thus, microfluidic applications are presented and discussed to solve some of these problems and to improve film qualities.
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    Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries
    (2019) Akentjew, T. L.; Terraza Inostroza, Claudio; Suazo, C.; Maksimuck, J.; Wilkens, C. A.; Vargas Rojas, Francisco Ignacio; Zavala, G.; Ocana, M.; Enrione, J.; Valenzuela Roediger, Loreto Margarita; García Herrera, C. M.; Blaker, J. J.; Khoury, M.; Acevedo, J. P.