Browsing by Author "Dinamarca Ceballos, Margarita Constanza."
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- ItemAmyloid-B-Acetylcholinesterase complexes potentiate neurodegenerative changes induced by the AB peptide. Implications for the pathogenesis of Alzheimer's disease(2010) Dinamarca Ceballos, Margarita Constanza.; Sagal, Juan P.; Quintanilla Gómez, Rodrigo Arthur; Godoy, Juan A.; Arrázola, Macarena S; Inestrosa Cantín, NibaldoAbstract The presence of amyloid-β (Aβ) deposits in selected brain regions is a hallmark of Alzheimer's disease (AD). The amyloid deposits have "chaperone molecules" which play critical roles in amyloid formation and toxicity. We report here that treatment of rat hippocampal neurons with Aβ-acetylcholinesterase (Aβ-AChE) complexes induced neurite network dystrophia and apoptosis. Moreover, the Aβ-AChE complexes induced a sustained increase in intracellular Ca2+ as well as a loss of mitochondrial membrane potential. The Aβ-AChE oligomers complex also induced higher alteration of Ca2+ homeostasis compared with Aβ-AChE fibrillar complexes. These alterations in calcium homeostasis were reversed when the neurons were treated previously with lithium, a GSK-3β inhibitor; Wnt-7a ligand, an activator for Wnt Pathway; and an N-methyl-D-aspartate (NMDA) receptor antagonist (MK-801), demonstrating protective roles for activation of the Wnt signaling pathway as well as for NMDA-receptor inhibition. Our results indicate that the Aβ-AChE complexes enhance Aβ-dependent deregulation of intracellular Ca2+ as well as mitochondrial dysfunction in hippocampal neurons, triggering an enhanced damage than Aβ alone. From a therapeutic point of view, activation of the Wnt signaling pathway, as well as NMDAR inhibition may be important factors to protect neurons under Aβ-AChE attack.
- ItemNeurobiological Effects of Hyperforin and its Potential in Alzheimer's Disease Therapy(2010) Griffith, T. N.; Varela Nallar, Lorena Patricia; Dinamarca Ceballos, Margarita Constanza.; Inestrosa Cantín, Nibaldo
- ItemStructure-Function Implications in Alzheimers Disease: Effect of Aβ Oligomers at Central Synapses(2008) Cerpa Nebott, Waldo Francisco; Dinamarca Ceballos, Margarita Constanza.; Inestrosa Cantín, Nibaldo
- ItemThe Synaptic Protein Neuroligin-1 Interacts with the Amyloid beta-Peptide. Is There a Role in Alzheimer's Disease?(2011) Dinamarca Ceballos, Margarita Constanza.; Inestrosa Cantín, Nibaldo