Browsing by Author "Delgado, Veronica"

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    A global point prevalence survey of antimicrobial use in neonatal intensive care units: The no-more-antibiotics and resistance (NO-MAS-R) study
    (2021) Prusakov, Pavel; Goff, Debra A.; Wozniak, Phillip S.; Cassim, Azraa; Scipion, Catherine E. A.; Urzua, Soledad; Ronchi, Andrea; Zeng, Lingkong; Ladipo-Ajayi, Oluwaseun; Aviles-Otero, Noelia; Udeigwe-Okeke, Chisom R.; Melamed, Rimma; Silveira, Rita C.; Auriti, Cinzia; Beltran-Arroyave, Claudia; Zamora-Flores, Elena; Sanchez-Codez, Maria; Donkor, Eric S.; Kekomaki, Satu; Mainini, Nicoletta; Trochez, Rosalba Vivas; Casey, Jamalyn; Graus, Juan M.; Muller, Mallory; Singh, Sara; Loeffen, Yvette; Tamayo Perez, Maria Eulalia; Ferreyra, Gloria Isabel; Lima-Rogel, Victoria; Perrone, Barbara; Izquierdo, Giannina; Cernada, Maria; Stoffella, Sylvia; Ekenze, Sebastian Okwuchukwu; de Alba-Romero, Concepcion; Tzialla, Chryssoula; Pham, Jennifer T.; Hosoi, Kenichiro; Consuegra, Magdalena Cecilia Calero; Betta, Pasqua; Hoyos, O. Alvaro; Roilides, Emmanuel; Naranjo-Zuniga, Gabriela; Oshiro, Makoto; Garay, Victor; Mondi, Vito; Mazzeo, Danila; Stahl, James A.; Cantey, Joseph B.; Monsalve, Juan Gonzalo Mesa; Normann, Erik; Landgrave, Lindsay C.; Mazouri, Ali; Avila, Claudia Alarcon; Piersigilli, Fiammetta; Trujillo, Monica; Kolman, Sonya; Delgado, Veronica; Guzman, Veronica; Abdellatif, Mohamed; Monterrosa, Luis; Tina, Lucia Gabriella; Yunis, Khalid; Belzu Rodriguez, Marco Antonio; Le Saux, Nicole; Leonardi, Valentina; Porta, Alessandro; Latorre, Giuseppe; Nakanishi, Hidehiko; Meir, Michal; Manzoni, Paolo; Norero, Ximena; Hoyos, Angela; Arias, Diana; Sanchez, Ruben Garcia; Medoro, Alexandra K.; Sanchez, Pablo J.
    Background: Global assessment of antimicrobial agents prescribed to infants in the neonatal intensive care unit (NICU) may inform antimicrobial stewardship efforts.
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    Modular and Molecular Optimization of a LOV (Light-Oxygen-Voltage)-Based Optogenetic Switch in Yeast
    (2021) Romero, Andres; Rojas, Vicente; Delgado, Veronica; Salinas, Francisco; Larrondo, Luis F.
    Optogenetic switches allow light-controlled gene expression with reversible and spatiotemporal resolution. In Saccharomyces cerevisiae, optogenetic tools hold great potential for a variety of metabolic engineering and biotechnology applications. In this work, we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system, an optogenetic switch based on photoreceptors from the fungus Neurospora crassa. We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family. Among the tested modules, the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV. Further, the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch. Finally, we assessed the effects of the plasmid copy number and promoter strength controlling the expression of the FUN-LOV and HAP-LOV components, and observed that when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems. Altogether, we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast and can additionally be applied to other systems.