Browsing by Author "Cohn-Inostroza, Nicolás A."

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    2D printing of functional wrinkled films by DOD technology: Evaluation of cytocompatibility, antibacterial and virucidal properties against corona virus model
    (Elsevier Ltd., 2025) Rodríguez-Umanzor, Fernando E.; Alarcón-Núñez, Matías N.; Sarabia Vallejos, Mauricio; Cohn-Inostroza, Nicolás A.; Martínez-Campos, Enrique; Cue-López, Raquel; Rodríguez-Hernández, Juan; Terraza Inostroza, Claudio; González-Henríquez, Carmen M.
    Wrinkled polymeric films fabricated via Drop-on-Demand (DOD) inkjet printing offer a significant advancement in biomedical applications, combining antibacterial, virucidal, and cytocompatible properties (multifunctional biomaterials). This scalable and cost-effective method enables precise deposition of polymeric materials, creating customizable micro-wrinkled surfaces with high spatial resolution. The ability to deposit multiple materials simultaneously supports the development of gradient or multifunctional coatings tailored for biomedical needs. Functional monomer-based inks with a crosslinking agent were applied onto UV-ozonized polycarbonate substrates, enhancing hydrophilicity for uniform material deposition. Controlled UV exposure, vacuum drying, and plasma treatments produced homogeneous wrinkled patterns, which can be adapted for infection control and tissue engineering. Chemical analyses confirmed the polymerization and structural integrity of the films, while rheological studies validated the inks’ printability without satellite droplet formation. Biological evaluations revealed strong antibacterial effects against Staphylococcus aureus and Escherichia coli and complete inactivation of HCoV-229E, a human coronavirus model, in DMAEMA-based samples. Samples containing AAc and HEMA reduced infected cells by ~80 %; however, no significant differences were identified between smooth and wrinkled samples regarding virucidal capacity. These films also demonstrated robust cytocompatibility, making them a cost-effective, multifunctional solution for critical healthcare challenges. This represents one of the few materials that selectively combine antibacterial and virucidal properties while supporting cell proliferation, offering immense potential for advanced biomedical devices.
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    Development of Soft Wrinkled Micropatterns on the Surface of 3D-Printed Hydrogel-Based Scaffolds via High-Resolution Digital Light Processing
    (2024) Sarabia-Vallejos, Mauricio A.; Romero De la Fuente, Scarlett; Cohn-Inostroza, Nicolás A.; Terraza Inostroza, Claudio Alberto; Rodriguez-Hernandez, Juan; González-Henríquez, Carmen M.
    The preparation of sophisticated hierarchically structured and cytocompatible hydrogel scaffolds is presented. For this purpose, a photosensitive resin was developed, printability was evaluated, and the optimal conditions for 3D printing were investigated. The design and fabrication by additive manufacturing of tailor-made porous scaffolds were combined with the formation of surface wrinkled micropatterns. This enabled the combination of micrometer-sized channels (100–200 microns) with microstructured wrinkled surfaces (1–3 μm wavelength). The internal pore structure was found to play a critical role in the mechanical properties. More precisely, the TPMS structure with a zero local curvature appears to be an excellent candidate for maintaining its mechanical resistance to compression stress, thus retaining its structural integrity upon large uniaxial deformations up to 70%. Finally, the washing conditions selected enabled us to produce noncytotoxic materials, as evidenced by experiments using AlamarBlue to follow the metabolic activity of the cells.