Browsing by Author "Carrasco Gálvez, Basilio Alejandro"
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- ItemAdvances in breeding and biotechnology of legume crops(2016) Jacob, C.; Carrasco Gálvez, Basilio Alejandro; Schwember Neira, Andrés R.
- ItemAnalysis of anthocyanin biosynthesis genes expression profiles in contrasting cultivars of Japanese plum (Prunus salicina L.) during fruit development(2016) González, Máximo; Salazar, E.; Cabrera Medina, María Soledad; Olea Ortega, Pilar del Rosario; Carrasco Gálvez, Basilio Alejandro
- ItemApplication of inter-simple sequence repeats relative to simple sequence repeats as a molecular marker system for indexing blueberry cultivars(2013) Garriga, Miguel; Parra, Pablo A.; Caligari, Peter D. S.; Retamales, Jorge B.; Carrasco Gálvez, Basilio Alejandro; Lobos, Gustavo A.; García Gonzales, Rolando
- ItemAssociation between the concentration of n-alkanes and tolerance to cracking in commercial varieties of sweet cherry fruits(2015) Rios, Juan Carlos; Robledo, Francisco; Schreiber, Lukas; Zeisler, Viktoria; Lang, Erika; Carrasco Gálvez, Basilio Alejandro; Silva, Herman
- ItemCombining niche modelling, land-use change, and genetic information to assess the conservation status of pouteria splendens populations in Central Chile(2015) Morales, Narkis S.; Fernández, Ignacio C.; Carrasco Gálvez, Basilio Alejandro; Orchard, Cristina
- ItemConstruction of a highly saturated linkage map in Japanese plum (Prunus salicina L.) using GBS for SNP marker calling(2018) Carrasco Gálvez, Basilio Alejandro; González, Máximo; Gebauer H., Marlene; García González, Rolando; Maldonado, Jonathan; Silva, Hernan
- ItemDe novo transcriptome assembly of 'Angeleno' and 'Lamoon' Japanese plum cultivars (Prunus salicina)(2016) González, Máximo; Maldonado, Jonathan; Salazar, Erika; Silva, Herman; Carrasco Gálvez, Basilio Alejandro
- ItemDevelopment and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)(2018) Carrasco Gálvez, Basilio Alejandro; Garcia Gonzales, Rolando.; Pico Mendoza, José.; Quiroz, Karla.; Cáceres, Pablo.; Chong Perez, Borys.; Pino, Hugo.; Seiltgens, Marjorie.; Greck, Eglis.; Caligari, Peter D. S.Abstract Background Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Results Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. Conclusions From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.
- ItemDiversidad genética del ajo chilote (Allium ampeloprasum L.) cultivado en la Isla de Chiloé, Chile(2022) Medina Huaman, Yenny Paola; Carrasco Gálvez, Basilio Alejandro; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalEl ajo chilote (Allium ampeloprasum L) es una especie hexaploide, infértil, que es propagado a través de bulbos. En Chile, es un cultivo tradicional que representa el 5% de la producción nacional de ajo, siendo cultivado principalmente en el sur del país. En el 2019 se realizó una recolección de 23 accesiones en campos de pequeños agricultores del sur de Chile con el fin de estudiar la diversidad genética de este cultivo. Para ello se utilizaron 20 marcadores moleculares tipo microsatélite, 11 derivados de secuencias expresadas (ESTs-SSR) y 9 de origen genómico (nSSR). Once de los 20 marcadores presentaron amplificación permitiendo determinar la existencia de 41 alelos en la población, con un promedio de cuatro alelos por locus. Los índices de diversidad Ho y He fueron 0,57 y 0,58 respectivamente, el PIC fue de 0,47. Las 266 muestras fueron agrupadas en 15 genotipos multilocus. El análisis de coordenadas principales y el análisis jerárquico de clúster utilizado para determinar las relaciones genéticas entre las accesiones, mostraron la existencia de tres grupos. El análisis de estructura genética detectó la presencia de dos grupos genéticos. Los resultados nos permiten indicar la existencia de una baja diferenciación genética en la población de A. ampeloprasum cultivado en la Isla de Chiloé siendo todos los individuos genéticamente muy homogéneos. Entre los 15 genotipos identificados existe uno predominante que podría ser caracterizado morfológica y agronómicamente y ser propuesto como una variedad local. La diversidad encontrada en esta población podría ser mantenida conservando 15 individuos que representen a los 15 multilocus.
- ItemEcosystem services in certified and non-certified coffee agroforestry systems in Costa Rica(2020) Pico Mendoza, J.; Pinoargote, M.; Carrasco Gálvez, Basilio Alejandro; Andrade, R. L.
- ItemEstablishment of photomixotrophic cultures for raspberry micropropagation in Temporary Immersion Bioreactors (TIBs)(2013) Arencibia, Ariel D.; Vergara, Carolina; Quiróz, Karla; Carrasco Gálvez, Basilio Alejandro; García Gonzales, Rolando
- ItemGenetic and morphological characterization of the endangered Austral papaya Vasconcellea chilensis (Planch. ex A. DC.) Solms(2014) Carrasco Gálvez, Basilio Alejandro; Garcia, R.; Diaz, C.; Avila, P.; Caceres, P.; Lobos Villalobos, Gabriel Antonio; Silva M., Hugo; Caligari, P.
- ItemGenetic characterization of japanese plum cultivars (prunus salicina) using SSR and ISSR molecular markers(2012) Carrasco Gálvez, Basilio Alejandro; Díaz Gálvez, Carole; Roas Moya, Mario Alejandro; Gebauer H., MarleneThe genetic characterization of 29 elite Japanese plum cultivars (Prunus salicina) and 4 Prunus cultivars was carried out by analyzing 97 Simple Sequence Repeat (SSR) alleles and 232 binary Inter Simple Sequence Repeat (ISSR) loci. A high level of genetic variability was found for these two molecular markers among the Japanese plum cultivars compared to other Prunus species. On average, the variability found by analyzing the SSR alleles were Na = 12.1, Ne = 5.2, Ho = 0.9, He = 0.8 and F= -0.127, whereas ISSR yielded values of h = 0.15 and I = 0.27. The genetic relationship among cultivars was estimated with Principal Coordinate Analysis (PCA) and a Bayesian clustering approach using the software program Structure. This program identified two subgroups (k=2). The first group included cultivars of four Prunus species: P. salicina, P. armeniaca, P. domestica and P. ceracifera, whose memberships ranged between 0.74 and 1.0. The second group included 19 Japanese plum cultivars and one plumcot cultivar, with memberships between 0.57 and 0.99. With some exceptions, similar relationships among cultivars were foundPCA. The level of genetic differentiation between two groups was low (Gst =0.055 and ST =0.04), and a low level of linkage disequilibrium (LD) was observed for all allele combinations. These results suggest that the high level of genetic variability, the low level of LD and the scarce degree of differentiation detected by Structure between the two genetic groups can be explained by the self-incompatibility mechanism that favors the exchange between genetically distant Prunus cultivars and by the intra- and interspecific hybridization strategies frequently used in plum breeding programs.
- ItemGenetic diversity and intra-racial structure of Chilean Choclero corn (Zea mays L.) germplasm revealed by simple sequence repeat markers (SSRs)(2017) Carrasco Gálvez, Basilio Alejandro
- ItemGenetic structure based on EST–SSR: a putative tool for fruit color selection in Japanese plum (Prunus salicina L.) breeding programs(2016) Gonzalez, M.; Salazar, E.; Castillo, J.; Morales, P.; Mura, I.; Maldonado, J.; Silva M., Hugo; Carrasco Gálvez, Basilio Alejandro
- ItemGenetic, cytological and molecular characterization of chia (Salvia hispanica L.) provenances(2017) Palma Rojas, C.; Gonzalez, C.; Carrasco Gálvez, Basilio Alejandro; Silva M., Hugo; Silva Robledo, H.
- ItemGenome-wide identification and characterization of R2R3MYB family in Rosaceae(2016) Gonzalez, M.; Carrasco Gálvez, Basilio Alejandro; Salazar, E.
- ItemHeterogeneous genetic structure in a natural population of raulí (Nothofagus nervosa).(2011) Carrasco Gálvez, Basilio Alejandro; Eaton, Lafayette; Letelier, Luis; Díaz Gálvez, Carole; García-Gonzáles, Rolando
- ItemIn vitro culture of Luma chequen (Mol) A. Gray from vegetative buds(2013) Mancilla, H.; Quiroz, K.; Arancibia, A.; Carrasco Gálvez, Basilio Alejandro; García Gonzáles, R.
- ItemIn vitro culture of Luma chequen from vegetative buds = Establecimiento al cultivo in vitro de Luma chequen a partir de segmentos nodales.(2013) Mancilla Chávez, Héctor Eduardo; Quiroz, Karla; Arencibia, Ariel; Carrasco Gálvez, Basilio Alejandro; García-Gonzáles, Rolando