Browsing by Author "Carneiro, Maria Fernanda Hornos"

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    Effects of native forest and human-modified land covers on the accumulation of toxic metals and metalloids in the tropical bee Tetragonisca angustula
    (2021) Barbosa, Marcela de Matos; Fernandes, Ana Carolina Coelho; Alves, Rafael Souza Cruz; Alves, Denise Araujo; Barbosa Junior, Fernando; Batista, Bruno Lemos; Ribeiro, Milton Cezar; Carneiro, Maria Fernanda Hornos
    The intensive shift on land cover by anthropogenic activities have led to changes in natural habitats and environmental contamination, which can ultimately impact and threat biodiversity and ecosystem services, such as pollination. The aim of this study was to evaluate the effect of native forest and human-modified land covers on the concentrations of chemical elements accumulated in the neotropical pollinator bee T. angustula. Eight landscapes, within an Ecological Corridor in the State of Sao Paulo, Brazil, with gradients of forest cover, spatial heterogeneity and varying land covers were used as sampling unities. Bees collected in traps or through actives searches had the concentration of 21 chemical elements determined by ICP-MS. Results show a beneficial effect of forested areas on the concentrations of some well-known toxic elements accumulated in bees, such as Hg, Cd, and Cr. Multivariate Redundancy Analysis (RDA) suggests road as the most important driver for the levels of Cr, Hg, Sb, Al, U, As, Pb and Pt and bare soil, pasture and urban areas as the landscape covers responsible for the concentrations of Zn, Cd, Mn, Mg, Ba and Sr in bees. The results reinforce the potential use of T. angustula bees as bioindicators of environmental quality and also show that these organisms are being directly affected by human land use, offering potential risks for the Neotropical ecosystem. Our study sheds light on how land covers (native forest and human-modified) can influence the levels of contaminants in insects within human-dominated landscapes. The generation of predictions of the levels of toxic metals and metalloids based on land use can both contribute to friendly farming planning as well as to support public policy development on the surrounding of protected areas and biodiversity conservation hotspots.
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    Exploring the impact of Agaricus bisporus on mitigating lead reproductive toxicity using the Caenorhabditis elegans model
    (2024) Caetano, Erika Leao Ajala; Miguel, Francisco Javier Novoa San; Leon, Rocio Errazuriz; Grotto, Denise; Carneiro, Maria Fernanda Hornos
    Given that Agaricus bisporus, an edible mushroom, has demonstrated antioxidant properties, our investigation aimed to assess whether Agaricus bisporus could mitigate the toxic effects of lead (Pb) on Caenorhabditis elegans (C. elegans) model. A dose-response study was conducted involving Pb and Agaricus bisporus to determine appropriate doses. Subsequently, a co-exposure study utilizing C. elegans strains N2 and CL2166 was implemented, with groups designated as Control, Pb, Agaricus bisporus, and Pb + Agaricus bisporus. Our findings revealed that co-exposure to Pb + 100 mg/mL Agaricus bisporus resulted in reduced embryonic and larval lethality, increased brood size, and enhanced motility compared to nematodes exposed solely to Pb. Notably, our observations indicated a transfer of reproductive toxicity from nematode parents to their offspring. Thus, Agaricus bisporus may play a significant role in Pb detoxification, suggesting its potential as a natural antioxidant for neutralizing the detrimental effects of Pb on reproductive health.
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    Photoaged polystyrene nanoplastics exposure results in reproductive toxicity due to oxidative damage in Caenorhabditis elegans
    (2024) Leon, Rocio Errazuriz; Salcedo, Vicente Andre Araya; Miguel, Francisco Javier Novoa San; Tardio, Cynthia Rosa Andrea Llanquinao; Briceno, Adolfo Andres Tobar; Fouilloux, Stefano Francesco Cherubini; Barbosa, Marcela de Matos; Barros, Cesar Antonio Saldias; Waldman, Walter Ruggeri; Espinosa-Bustos, Christian; Carneiro, Maria Fernanda Hornos
    The increase of plastic production together with the incipient reuse/recycling system has resulted in massive discards into the environment. This has facilitated the formation of micro- and nanoplastics (MNPs) which poses major risk for environmental health. Although some studies have investigated the effects of pristine MNPs on reproductive health, the effects of weathered MNPs have been poorly investigated. Here we show in Caenorhabditis elegans that exposure to photoaged polystyrene nanoplastics (PSNP-UV) results in worse reproductive performance than pristine PSNP (i.e., embryonic/larval lethality plus a decrease in the brood size, accompanied by a high number of unfertilized eggs), besides it affects size and locomotion behavior. Those effects were potentially generated by reactive products formed during UV -irradiation, since we found higher levels of reactive oxygen species and increased expression of GST-4 in worms exposed to PSNP-UV. Those results are supported by physical -chemical characterization analyses which indicate significant formation of oxidative degradation products from PSNP under UV -C irradiation. Our study also demonstrates that PSNP accumulate predominantly in the gastrointestinal tract of C. elegans (with no accumulation in the gonads), being completely eliminated at 96 h post -exposure. We complemented the toxicological analysis of PSNP/PSNP-UV by showing that the activation of the stress response via DAF-16 is dependent of the nanoplastics accumulation. Our data suggest that exposure to the wild PSNP, i.e., polystyrene nanoplastics more similar to those actually found in the environment, results in more important reprotoxic effects. This is associated with the presence of degradation products formed during UV -C irradiation and their interaction with biological targets.
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    Removal of chlorpyrifos from water using biosorbents derived from cassava peel, crambe meal, and pinus bark
    (2022) Schwantes, Daniel; Goncalves Jr, Affonso Celso; Fuentealba, Denis; Carneiro, Maria Fernanda Hornos; Tarley, Cesar Ricardo Teixeira; Prete, Maiyara Carolyne